Cell culture
Human MCF-10A, human retinal pigment epithelial (RPE-1), human lung fibroblasts (HLFs) and transformed human embryonic kidney (HEK293T) cells were obtained from ATCC. MEFs were gift from J. Vidigal. Cell lines not obtained from ATCC…
Category: 5. Biology
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Identification and structural characterization of pseudogenes in Fusarium graminearum
Balakirev, E. S., Ayala, F. J. & Pseudogenes Are they junk or functional DNA? Annu. Rev. Genet. 37, 123–151 (2003).
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Tutar, Y. Pseudogenes. Comp. Funct. Genomics. 424526 (2012).
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Text-related functionality and dynamics of visual human pre-frontal activations revealed through neural network convergence
Grill-Spector, K. & Malach, R. The human visual cortex. Annu. Rev. Neurosci. 27, 649–677 (2004).
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Noy, N. et al. Ignition’s glow: ultra-fast spread of global cortical activity…
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Programmable protein ligation on cell surfaces
General materials
Common reagents and chemicals were purchased from MilliporeSigma unless stated otherwise. The 2,4-dinitrochlorobenzene (CAS number 97-00-7), tert-butyl bromoacetate (CAS 5292-43-3), N-hydroxysuccinimide (CAS 6066-82-6),…
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Damage to white matter networks resulting from small vessel disease and the effects on cognitive function
Christiansen, P., Larsson, H. B. W., Thomsen, C., Wieslander, S. B. & Henriksen, O. Age dependent white matter lesions and brain volume changes in healthy volunteers. Acta radiol. 35(2), 117–122. https://doi.org/10.1177/028418519403500203…
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Direct somatic embryogenesis induction in Aspilia Africana (Pers.) C. D. Adams, and assessment of genetic homogeneity and physiology of regenerants
Plant material and Preparation of explants
With permission from local authorities, A. africana seeds (mature and dry) were collected and provided by Natural Chemotherapeutics Research Institute (NCRI) from about 50 healthy plants growing at Pece,…
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Respiratory viral infections awaken metastatic breast cancer cells in lungs
Mouse strains, influenza infection and antibody treatments
Transgenic mouse models of breast cancer, using mouse mammary tumour virus (MMTV) long terminal repeats, are widely used. In brief, MMTV-PyMT and MMTV-erbB2/neu/Her2 (MMTV-Her2) mice…
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Caerin 1.1/1.9 interfere KHDRBS1-DDX5 regulatory axis to induce IL-18 mediated pyroptosis in a HeLa cell tumour model
Cells
The HeLa cell was obtained from the Shanghai Institute for Biological Sciences, Chinese Academy of Sciences. The GSDME KO HeLa cell line was provided by Dr Feng Shao (Chinese Academy of Biological Sciences, Beijing). The KHDRBS1 KO and DDX5…
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Assessing alpha lattice design for heat stress indices and yield stability in wheat genotypes
Assessment of heat stress indices
Heat stress indices are quantitative tools, widely used to examine the impacts of heat stress on plant growth and development28. Stress indices are crucial tools for assessing physiological and yield-related…
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CRISPR-GPT for agentic automation of gene-editing experiments
Building AI co-pilot harnessing LLM’s reasoning ability
CRISPR-GPT supports four major gene-editing modalities and 22 gene-editing experiment tasks (Fig. 1 and Supplementary Table 1). It offers tunable levels of automation via three modes: Meta,…
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