Category: 5. Biology

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  • Molecular basis of TRPV3 channel blockade by intracellular polyamines

    Molecular basis of TRPV3 channel blockade by intracellular polyamines

  • Igarashi, K. & Kashiwagi, K. The functional role of polyamines in eukaryotic cells. Int J. Biochem. Cell Biol. 107, 104–115 (2019).

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  • Bekebrede, A. F. et al. The…

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  • RNA transcripts serve as a template for double-strand break repair in human cells

    RNA transcripts serve as a template for double-strand break repair in human cells

    Cell culture

    HEK293T (ATCC, #CLR-3216) cells were routinely grown with Dulbecco’s Modified Eagle’s Medium media supplemented with 10% bovine calf serum, 100 U/mL Penicillin-Streptomycin, 1% non-essential amino acids, 2mM l-glutamine. Cells…

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  • SUMO2/3 modification of transcription-associated proteins controls cell viability in response to oxygen and glucose deprivation-mediated stress

    SUMO2/3 modification of transcription-associated proteins controls cell viability in response to oxygen and glucose deprivation-mediated stress

    Cell culture, transfection, and generation of stable cell lines

    P19 cells were directly obtained from ATCC (LGC Standards, Barcelona, Spain) as authenticated. They were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, St….

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  • Kinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidase

    Kinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidase

    Ethical approvals

    The study and all animal procedures were performed according to relevant guidelines and regulations and following protocols approved under ethical permits 1666/19 and 38/23 granted by the Gothenburg Regional Animal Ethics…

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  • Let-7 restrains an epigenetic circuit in AT2 cells to prevent fibrogenic intermediates in pulmonary fibrosis

    Let-7 restrains an epigenetic circuit in AT2 cells to prevent fibrogenic intermediates in pulmonary fibrosis

    Time-dependent downregulation of let-7 family expression during peak formation of ADIs after bleomycin-induced lung injury

    We examined let-7 expression dynamics after bleomycin-induced lung injury in mice (GSE195773)25. Our analysis of this…

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  • DNA glycosylase (NEIL3) overexpression associated with low tumor immune infiltration and poor overall patient survival in endometrial cancer

    DNA glycosylase (NEIL3) overexpression associated with low tumor immune infiltration and poor overall patient survival in endometrial cancer

  • Society, A. C. Ket statisticis for rndometrial cancer. (2019).

  • Amant, F. et al. Endometrial cancer. Lancet 366, 491–505. https://doi.org/10.1016/S0140-6736(05)67063-8 (2005).

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  • Time-course liver microarray data from 0 to 14 days in a 70% partial hepatectomy mouse model

    Time-course liver microarray data from 0 to 14 days in a 70% partial hepatectomy mouse model

    70% PH

    All animal experiments were performed in accordance with the Nihon University Animal Care and Use Committee and Nihon University Rules concerning Animal Care and Use. C57BL/6 N mice were purchased from the Charles River Laboratories. 70%…

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  • Cellular immune signatures and differences of four porcine circovirus type 2 vaccines to heterologous PCV2d infection

    Cellular immune signatures and differences of four porcine circovirus type 2 vaccines to heterologous PCV2d infection

    PCV2-ORF2-specific IFNγ-SC responses induced by different vaccines exhibit distinct kinetics

    A range of immunological assays was performed at indicated time points to comprehensively evaluate the cellular immune response in blood and tissues…

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  • BiDAC-dependent degradation of plasma membrane proteins by the endolysosomal system

    BiDAC-dependent degradation of plasma membrane proteins by the endolysosomal system

    Cell line generation and culture conditions

    SKOV3 and SKBR3 cell lines were cultured at 37 °C with 5% CO2 in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and Glutamax (Gibco). Parental SKOV3 and SKBR3 lines were obtained from…

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