These authors contributed equally: Apostolos Zournas, Matthew R. Incha.
Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA
Apostolos Zournas, Matthew R. Incha, Tijana Radivojevic, Vincent…
Category: 5. Biology
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ATP functions as a pathogen-associated molecular pattern to activate the E3 ubiquitin ligase RNF213
Protein sequence analysis, cloning of mouse RNF213 expression constructs, expression and purification of mouse RNF213 protein, preparation of fluorescently labeled ubiquitin, the RNF213 SDS-PAGE-based auto-ubiquitination assay, cryo-EM analyses,…
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Cell atlases for reproductive health
To this end, two recent papers published in Nature Medicine report cell atlases created from women with reproductive disorders. A study from the University of Tartu, led by Triin Laisk, conducted genome-wide association studies for 42 health…
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Synergistic effect of synthetic derivatives of 1,3,4-thiadiazole with amphotericin B in antifungal therapy
Study of antifungal activity
The structures of the 1,3,4-thiadiazole derivatives tested are presented in Fig. 1. The results illustrating the activity of individual substances against Candida strains are presented in Fig. 2. Among the 10…
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CTCF depletion decouples enhancer-mediated gene activation from chromatin hub formation
Cell culture
BLaER1 (ref. 45) and BLaER1-CTCF-mAID47 cells were cultured in RPMI 1640 medium (Gibco, 31870025), supplemented with 10% FBS (Gibco, 10270106), 25 mM HEPES (Fisher Scientific, 15-630-080) and 2% GlutaMAX Supplement (Thermo…
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Age dependent susceptibility and immune responses to La Crosse virus infection in non-human primates
Animals, ethics and clinical analysis
All infectious work with NHPs in this study was conducted with approval and oversight from the Rocky Mountain Laboratories (RML) Institutional Biosafety Committee and the RML Animal Care and Use Committee…
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A novel approach for synthesizing silver nanoparticles with antibacterial and cytotoxic activities using the leaf extract of hydroponically grown Moringa oleifera
In recent years, the rate of infectious diseases has increased rapidly due to microbial infections and the emergence of antibiotic drug resistance, which poses significant challenges and affects the overall quality of human health. The occurrence…
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Mycorrhizal CLE mimicry | Nature Plants
Since the discovery of systemin in tomato in the past century, small peptides have become ubiquitous in plant signalling pathways. Various families with increasingly funny names each contain dozens of individual peptides: PEP, RALF, CEP, IDA,…
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DiffInvex identifies evolutionary shifts in driver gene repertoires during tumorigenesis and chemotherapy
Comparing 8591 whole-genome sequenced tumors from pre-treated versus treatment-naive patients
To identify the genes under differential positive selection associated with chemotherapy treatment, we collected the somatic single-nucleotide variant…
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