Category: 5. Biology

Wild-type mice show avoidance and aversive facial expressions to object touch but not to social touch

We first asked if WT mice perceive social touch differently from non-social touch. We used the same behavioral assay we recently developed²¹ in…

Sample collection

Samples of industrial effluents were collected from Nacharam district, Hyderabad, India (“17.430800 N 78.559500 E”). The selected site encompasses many services and gas stations containing high levels of oil contamination. A…

Microbial analysis of dominant microbes

Figure 1a, b showed the microbial composition of pickled and salted chili peppers at genus level on days 30 and 45. Four non-fermentative microbes—Raoultella, Serratia, Pseudomonas, and Pectobacterium,…

The present results indicate that MSEW provoked progressive retinal alterations and that microglial activation could be one of the key factors linking the long-term consequences in retinal function and structure with MSEW. It has been clearly…

Design GAL4-derived PRCIS

As a proof-of-concept, we embarked on the rational engineering of a conformationally constrained GAL4, in which the DNA binding was incapacitated from structural distortion and could be reinstated by proteolytic cleavage….

Locations Push-V (1), Feof (2), Lis (3), and Bort (4)

At the initial research stage, we studied the first four locations Push-V, Feof, Lis, and Bort of the Kyiv agglomeration of S. commune. The principal component analysis by synthesized amplicons…

Defining the ancestral sequence

A reliable definition of mutation changes relies on the accurate definition of the ancestral state of each base in the genome of interest. To achieve this, we used CACTUS³⁴ which can generate ancestral genomes from…

Functional variant rs3101339 located in 1p31.1 with its risk allele C upregulating NEGR1 expression

We previously identified the functional SNP rs3101339 interrupting REST binding in 1p31.1 by integrating ChIP-seq data and position weight matrix [

Yeast strain and reagents

Saccharomyces cerevisiae strain EBY100 was used for yeast surface display.³⁵ Yeast strains were cultured in YPD media, which consisted of 10 g/L yeast extract, 20 g/L peptone, and 20 g/L dextrose. Solid media for yeast…