The amount of total cholesterol in the lysates was measured with the Amplex® Red Cholesterol Assay Kit (Beyotime, S0211M) following manufacturer’s instructions.
Flow cytometry analysis
Single cell suspensions were prepared in DPBS containing 2%…
Category: 5. Biology
-
Acetylation profiling by Iseq-Kac reveals insights into HSC aging and lineage decision
Zhong, Q. et al. Protein posttranslational modifications in health and diseases: functions, regulatory mechanisms, and therapeutic implications. MedComm (2020) 4, e261 (2023).
Google…
Continue Reading
-
Methods for multiplexing single-cell multi-omics
Baysoy, A., Bai, Z., Satija, R. & Fan, R. Nat. Rev. Mol. Cell Biol. 24, 695–713 (2023).
Google Scholar
Lobato-Moreno, S. et al. Nat Methods
Continue Reading
-
Base editing as a therapeutic strategy for somatic repeat expansion diseases
Base editing as a therapeutic strategy for somatic repeat expansion diseases
Continue Reading
-
An engineered U7 small nuclear RNA scaffold greatly increases ADAR-mediated programmable RNA base editing
Expression of ADAR gRNAs in a SmOPT U7 format boosts RNA editing
Guide RNAs for ADAR editing have traditionally been expressed by the U6 snRNA promoter, as for short hairpin RNAs and CRISPR gRNAs. We previously reported1,24 that a U6-driven…
Continue Reading
-
HaloPROTAC3 does not trigger the degradation of the halotagged parasitophorous vacuole membrane protein UIS4 during Plasmodium liver stage development
Cell culture
The human hepatoma Huh7 cell line (Japanese Collection of Research Bioresources (JCRB) Cell Bank) was cultured in Dulbecco’s Modified Eagle Medium (DMEM) with high glucose (Gibco) supplemented with 10% heat-inactivated fetal bovine…
Continue Reading
-
Stability and functional consequences of disulfide bond engineering in Aspergillus flavus uricase
Available uricase structures
A search for ‘uricase’ at the RCSB Protein Data Bank (PDB) yielded 121 experimentally resolved structures since 2000, reflecting the extensive research on this enzyme. Among these structures, we selected the X-ray…
Continue Reading
-
A multi-hospital, clinician-initiated bacterial genomics programme to investigate treatment failure in severe Staphylococcus aureus infections
Ballard, S. A., Sherry, N. L. & Howden, B. P. Public health implementation of pathogen genomics: the role for accreditation and application of ISO standards. Microb. Genom. 9, 001097 (2023).
Continue Reading
-
De novo induction of tertiary lymphoid structures: an immunotherapeutic strategy in pancreatic cancer
The recent publication by Amisaki et al.1 in Nature highlights a novel and druggable mechanism by which IL-33-activated innate lymphoid cells type 2 (ILC2s) induce the formation of tertiary lymphoid…
Continue Reading
