Identification of GCase-targeting nanobodies
To generate nanobodies (Nbs) specifically binding human lysosomal glucocerebrosidase (GCase), a llama was immunized with a commercial source of GCase (Velaglucerase, VPRIV®)43,44,45. To maximize the…
Category: 5. Biology
-
Plasma inflammation-related proteins associated with anxiety and depression disorders in IBD patients
This study characterized the clinical features of IBD patients with anxiety or depression and assessed their plasma inflammation-related proteins using OLINK technology. We found that active phase IBD patients exhibited significantly higher…
Continue Reading
-
Restoring calcium crosstalk between ER and mitochondria promotes intestinal stem cell rejuvenation through autophagy in aged Drosophila
Fly strains and reagents
Flies were cultured at 25 °C and 60% humidity with a 12 h light/dark cycle on standard yeast/molasses-based food unless otherwise indicated (recipe for 1 L food: 13.8 g agar, 22 g molasses, 75 g malt extract,…
Continue Reading
-
Production of multi-subunit proteins in CHO cells by transposase-mediated integration of subunit-splitting vectors
Cell lines
CHO-K1 cells were obtained from the European Collection of Cell Cultures (ECACC 85051005). CHO-K1 cells22 were adapted to suspension culture and maintained in a serum-free proprietary growth medium with 4 mM L-glutamine (Gibco) in shake…
Continue Reading
-
Expression of an interleukin-2 partial agonist enhances regulatory T cell persistence and efficacy in mouse autoimmune models
Plasmids and lentiviral vector production
Second-generation lentiviruses were utilized to generate human Tregs-IL2. The human Treg-IL2 transfer plasmids were designed and ordered from VectorBuilder’s website. These transfer plasmids contained…
Continue Reading
-
Transition and dynamic reconfiguration in late-life depression based on hidden Markov model
Blazer, D. G. Depression in late life: review and commentary. J. Gerontol. Ser. A Biol. Sci. Med. Sci. 58, M249–M265 (2003).
Google Scholar
Geerlings, M. I. & Gerritsen, L. Late-life…
Continue Reading
-
Comprehensive quantitative evaluation and mechanism analysis of influencing factors on yield and quality of cultivated Gastrodia elata Blume
The yield and quality of G. elata Bl.
The morphological features of cultivated G. elata Bl. from different environments were depicted in Fig. S1. For the yield of G. elata Bl., it was 13.22 kg/m2, 5.12 kg/m2, 11.80 kg/m2, 5.33 kg/m2, 14.00 kg/m2,…
Continue Reading
-
Saliva-derived transcriptomic signature for gastric cancer detection using machine learning and leveraging publicly available datasets
While saliva does not mirror the transcriptomic landscape observed in stomach tumorous tissue, it represents a promising non-invasive tool in GC screening and early detection.
Traditional tissue sampling for histopathological analysis offers the…
Continue Reading
-
Their personal paths in science
Their personal paths in science
Continue Reading
-
Grape polyphenols reduce fasting glucose and increase hyocholic acid in healthy humans: a meta-omics study
Participant demographics and effect of GP-SPI on CMP values, stool, and food intake patterns
This single-arm, 17-day longitudinal time course study (Fig. 2A) was carried out in accordance with the Declaration of Helsinki and was approved by the…
Continue Reading