Category: 5. Biology

Widely-used antidepressants, selective serotonin reuptake inhibitors (SSRIs), significantly increased the ability of T cells to suppress tumor growth in various cancer types in mouse and human tumor models…

A novel bird flu genotype is driving a surge in outbreaks across wild birds, poultry, and even a human in British Columbia, raising urgent questions about how this virus is evolving and what it means for future pandemic…

Ethical regulations

Our research complies with all relevant ethical regulations. The Boston Children’s Hospital Institutional Animal Care and Use Committee approved all mouse studies as per protocols 00002094 and 00002095. Deidentified…

Now, Yi-Lei Zhao, Mehdi Mobli and Xudong Qu report a polyheteroatomic Diels–Alderase that catalyses the intramolecular cycloaddition of a C=O diene with a C=O dienophile. The researchers had previously worked on the elucidation of the…

Expression and transcription analysis

Clinical and RNA-sequencing datasets from patients and normal controls were procured from The Cancer Genome Atlas (TCGA) database through the UCSC Xena platform (https://xena.ucsc.edu/), while normal control…

OATD-02 remodels tumor cell metabolism

To evaluate the metabolic impact of intracellular arginase inhibition by OATD-02, we analyzed the intracellular levels of key metabolites involved in L-arginine metabolism, including L-arginine, L-ornithine,…

Strain culture

B. theta ATCC29148 was thawed on Brain Heart Infusion agar plates (BHI, BD BBLTM) supplemented with 5 µg/ml hemin (Alfa Aesar) in 1 N NaOH and 2.5 µg/ml vitamin K (Thermo Fisher Scientific) in 100% EtOH (previously referred…

On the basis of the structure of TmPiT, which is homologous to the hPiT2 protein, we mapped the novel variant P631S and other variants in the TM8 helix, including V624E, W626 A, W626R, and S637R, to the hPiT2 TM domain (Fig. 2A).

1. Mohamed Nadhir Djekidel

   Present address: Center for Applied Bioinformatics, St. Jude Children’s Research Hospital, Memphis, TN, USA

2. These authors contributed equally: Zhiyuan Chen, Mohamed Nadhir Djekidel.

Authors and Affiliations

Acute lethal and teratogenicity assays

Artemia franciscana hatching

The hatching of A. franciscana cysts (White Mountain, Great Salt Lake Utah, USA) followed the protocol provided by the Aquarium del Puerto de Veracruz (formerly known as Acuario de…