Reagents
Colchicine, p38MAPK inhibitor III, paclitaxel, MG132, anacardic acid, cycloheximide, and propidium iodide were purchased from MedChemExpress (Monmouth Junction, NJ, U.S.A). Turbofect™ transfection reagent, Fetal bovine serum (F.B.S.),…
Category: 5. Biology
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NIBN Japan Microbiome Database, a database for exploring the correlations between human microbiome and health
Microbiome databases such as the Human Microbiome Project (HMP)14 and UK Biobank15 have set benchmarks for large-scale efforts to map the human microbiome across diverse populations, generating extensive genomic, metagenomic, and phenotypic data….
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Probing condensate microenvironments with a micropeptide killswitch
Generation of DNA constructs
A list of all of the oligonucleotides that were used to generate plasmids and used for quantitative PCR with reverse transcription (RT–qPCR) in this study is provided in Supplementary Table 1.
Generation of mammalian…
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Ciprofloxacin resistance rapidly declines in nfxB defective clinical strains of Pseudomonas aeruginosa
Santajit, S. & Indrawattana, N. Mechanisms of Antimicrobial Resistance in ESKAPE Pathogens. Biomed. Res Int 2016, 2475067 (2016).
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Rello, J. et…
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Maternal iron deficiency causes male-to-female sex reversal in mouse embryos
Kuroki, S. et al. Epigenetic regulation of mouse sex determination by the histone demethylase Jmjd1a. Science 341, 1106–1109 (2013).
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Kuroki, S….
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2D versus 3D tumor-on-chip models to study the impact of tumor organization on metabolic patterns in vitro
Demicco, M., Liu, X.-Z., Leithner, K. & Fendt, S.-M. Metabolic heterogeneity in cancer. Nat. Metab. 6, 18–38 (2024).
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Ge, H., Tian, M., Pei, Q., Tan, F. & Pei, H….
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Multiplexed single-molecule characterization at the library scale
Mohapatra, S., Lin, C.-T., Feng, X. A., Basu, A. & Ha, T. Single-molecule analysis and engineering of DNA motors. Chem. Rev. 120, 36–78 (2020).
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Bacic, L.,…
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Higher AJCC stage at primary tumor diagnosis may predict shorter survival in metastatic uveal melanoma
Descriptive statistics
Of the 333 included patients with metastatic disease, 176 (53%) were female. The mean age at the time of diagnosis of the primary tumor was 61 years (SD 12 years). The mean largest basal diameter (LBD) was 13.2 mm (SD…
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A FtsZ cis disassembly element acts in Z-ring assembly during bacterial cell division
Bacterial strains, plasmids, and cell culturing
All E. coli strains are described (Supplementary Table 4). All strains were derived from the E. coli strain BW25113, which has the following genotype: F–, DE(araD-araB)567, lacZ4787(del)::rrnB-3,…
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Molecular basis of the urate transporter URAT1 inhibition by gout drugs
Consensus mutagenesis design
Consensus constructs were designed in a similar manner to what has been previously reported19,27, with minor modifications. First, PSI-BLAST was performed to identify 250 hits from UniProt database using human…
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