Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate…
Category: 5. Biology
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Impact of climate change on the distribution of Ligustrum leucanthum using MaxEnt modeling
Kufa, C. A., Bekele, A. & Atickem, A. Impacts of climate change on predicted habitat suitability and distribution of Djaffa mountains Guereza (Colobus guereza gallarum, Neumann 1902) using MaxEnt algorithm in eastern Ethiopian highland. Glob….
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Turning gene expression up and down
CRISPR-based technologies facilitate genome manipulation to understand the function of specific elements and have even entered the clinic to treat disease via modulation of gene expression. Existing approaches to screen regulatory effects in the…
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Deep-sea-floor diversity in Asteroidea is shaped by competing processes across different latitudes and oceans
Global patterns of species richness
At a global scale, species richness peaks ~10° N of the Equator in shallow tropical waters (Fig. 2a,c) with a strong decline in species number throughout temperate waters on both sides of the Equator,…
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Time-varying stimuli that prolong IKK activation promote nuclear remodeling and mechanistic switching of NF-κB dynamics
IL-1 pulse duration produces a monotonic correlation between same-cell IKK and NF-κB responses
We previously developed a CRISPR-modified U2OS cell line that co-express FP fusions of NEMO (EGFP-NEMO) and RelA (mCh-RelA) from their endogenous loci….
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Suicide gene therapy targeting ewing sarcoma via an ewing-specific GGAA promoter
Cell lines
The Ewing sarcoma cell line A673 (CRL-1598), the fibrosarcoma cell line HT1080 (CCL-121) and the osteosarcoma cell lines U2-OS (HTB-96) and Saos-2 (HTB-85) were purchased from the American Type Culture Collection (Manassas, VA, USA)….
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A call for funds and training for kidney genomics programmes
In countries with resource constraints, lack of access to genetic testing leads to under-diagnosis of genetic kidney diseases, resulting in a missed opportunity to initiate treatment to prevent kidney failure. Funding, education and engagement…
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Accelerated wound healing through tannin-rich Jaft extract, concentration-dependent efficacy and mechanistic insights from Quercus brantii ointment formulations
Reactive oxygen species (ROS) play a dual role in wound healing, where both excessive production and inadequate detoxification can lead to oxidative stress, exacerbating inflammation and delaying tissue repair12. This underscores the importance…
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Structure and function of the human apoptotic scramblase Xkr4
Full length hXkr4 scrambles lipids
To test whether Xkr proteins are scramblases we sought to purify and functionally reconstitute them in proteoliposomes. A screen of GFP-tagged family members using fluorescence size exclusion chromatography…
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The therapeutic potential of pegylated arginase I treatment in glioblastoma
Cell cultures
GBM cell lines U87 (HTB-14), U373 (HTB-19), and U138 (HTB-16) were purchased from American Type Culture Collection (Virginia, USA). D54 was a gift from Duke University Medical Center, Durham, NC, USA23. The mouse microglia cell line…
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