Category: 5. Biology

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  • Testing the limits of short-reads metagenomic classifications programs in wastewater treating microbial communities

    Testing the limits of short-reads metagenomic classifications programs in wastewater treating microbial communities

    Mock processing stats

    After BBDuk filtering, 46,315,875 out of 50,001,759 paired reads (92.6%) remained available for analysis. Kaiju classified between 94% (E-value 0.01 and minimal alignment length “m” = 11) and 76% of these sequences…

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  • Letermovir reduces cytomegalovirus in HLA-matched and mismatched allogeneic hematopoietic cell transplant patients receiving post-transplant cyclophosphamide

    Letermovir reduces cytomegalovirus in HLA-matched and mismatched allogeneic hematopoietic cell transplant patients receiving post-transplant cyclophosphamide

    Competing interests

    RF is a member of the advisory board for Kite/Gilead and Autolus; receives research funding from Novartis and Orca Bio; and provides consultancy and honoraria for Sanofi. AM receives…

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  • A modular fluorescent camera unit for wound imaging

    A modular fluorescent camera unit for wound imaging

    The imaging unit, a modular fluorescent camera system, has been designed for flexible assembly and operation. Figure 1a shows the primary use case for the imaging unit, where a wound image is captured and analyzed to estimate features like the…

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  • SUMOylation is not a prerequisite for HSF1’s role in stress protection and transactivation

    SUMOylation is not a prerequisite for HSF1’s role in stress protection and transactivation

    DNA construct

    All HSF1 mutants were derived from the human HSF1 sequence (NM_005526.4) and cloned into the pDONR221 vector using the Gateway cloning system (Thermo Fisher Scientific Inc., Waltham, MA, USA). SBP-SUMO1 and SBP-SUMO2 constructs were…

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  • Multiomics and cellular senescence profiling of aging human skeletal muscle uncovers Maraviroc as a senotherapeutic approach for sarcopenia

    Multiomics and cellular senescence profiling of aging human skeletal muscle uncovers Maraviroc as a senotherapeutic approach for sarcopenia

    Multiomics mapping of senescence atlas in human muscle

    To gain the first senescence blueprint of mononucleated cells in aging human muscle, we obtained hamstring muscles from biopsies of 10 male donors: five young (19–27 years-old males who…

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  • Comparative genome analyses reveal insights into the marine adaptation of a novel bacterium Pontimicrobium maritimus sp. nov., isolated from seawater

    Comparative genome analyses reveal insights into the marine adaptation of a novel bacterium Pontimicrobium maritimus sp. nov., isolated from seawater

    Novel strain isolation and morphology

    As a part of the Korean West Sea microbial diversity project, strain SW4T was isolated from seawater. The isolate grew optimally on MA, producing yellow, round colonies. SEM images revealed that the cells of…

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  • Lactose fermenting enteroinvasive Escherichia coli from diarrhoeal cases confers enhanced virulence

    Lactose fermenting enteroinvasive Escherichia coli from diarrhoeal cases confers enhanced virulence

  • Farajzadeh-Sheikh, A. et al. Distribution of genes encoding virulence factors and the genetic diversity of enteroinvasive Escherichia coli (EIEC) isolates from patients with Diarrhea in Ahvaz. Iran. Infect. Drug Resist. 13, 119–127 (2020).

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  • Gut microbiota profiles of peninsular Malaysian populations are associated with urbanization and lifestyle

    Gut microbiota profiles of peninsular Malaysian populations are associated with urbanization and lifestyle

    Participant demographics and lifestyles

    A total of 87 participants were recruited for this study as part of the Global Microbiome Conservancy (http://microbiomeconservancy.org) across three localities: Gua Musang (n = 20), Tasik Banding…

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  • Harnessing protein language model for structure-based discovery of highly efficient and robust PET hydrolases

    Harnessing protein language model for structure-based discovery of highly efficient and robust PET hydrolases

    Structure search

    In the structural search step, we utilized the default setting of the FoldSeek server website (https://search.foldseek.com/search). The structure of IsPETase (PDB: 5XFY) is selected as the only query protein for the FoldSeek…

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