Category: 5. Biology

Ethics

Experiments using transgenic Drosophila melanogaster stocks were approved under Chiba University Institutional Biosafety Committee protocol #G5-46.

Fly strains and keeping conditions

A cohort of 104 strains derived from the DGRP was used in…

Tissue collection

Tumor tissues were collected from 32 patients with BC who underwent tumorectomy (Supplementary Table 2). The use of all the patient material was approved by the Ethics Committee of Tongji Hospital, Tongji Medical College,…

“The low implantation efficiency of human stem cells in the host’s aging microenvironment, their short functional residence time, and the potential risk of tumorigenesis together constitute the technical bottlenecks that restrict the…

Data generation workflow

Using in-house scripts, we created a workflow to date patient gene (accession number) to go from cDNA sequence (NCBI) and generate all missense variants encoded for that cDNA, which in the case of PMM2 is 1454 variants….

Landmark description

Applying geometric morphometrics to human facial shape involves the precise use of landmarks to capture facial morphology. Ideally, landmarks should denote key morphological features as discrete, anatomically homologous points…

Global public health exigency in improving the sensitivity of parasite detection

Intestinal parasites reside in the human intestine. Among the wide variety of species, more than 300 types of parasites can be transmitted to human⁵, including but…

Animal models

Animal experiments were in accordance with the Institutional Animal Care and Use Committee (IACUC, protocols 202100001/2) approved by the New York University Grossman School of Medicine. All mice were on a C57BL/6 J background and…

Data acquisition and data preprocessing

The experiment selected a reserve breeding pigsty from a large-scale breeding farm located around Hohhot, Inner Mongolia Autonomous Region, China, for data collection. The pigsty measures 5.3 × 2.7 m…

Experiment design and respiratory sample characteristics

Thirty-five BALF samples and 34 paired OP samples were collected from 35 patients, including 26 with pneumonia and 9 with other diseases (Supplementary Table 1). Negative controls,…