Category: 5. Biology

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  • RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteria

    RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteria

    S. pyogenes culture and mutagenesis

    Streptococcus pyogenes strain SF370; M1 GAS and its derivatives were cultured on Trypticase soy agar (BD Difco) plates supplemented with 5% defibrinated sheep blood. In THY, C medium and…

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  • Neurogenomic and behavioral principles shape freezing dynamics and synergistic performance in Drosophila melanogaster

    Neurogenomic and behavioral principles shape freezing dynamics and synergistic performance in Drosophila melanogaster

    Ethics

    Experiments using transgenic Drosophila melanogaster stocks were approved under Chiba University Institutional Biosafety Committee protocol #G5-46.

    Fly strains and keeping conditions

    A cohort of 104 strains derived from the DGRP was used in…

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  • NR4A3 potentials M1-like macrophage polarization to facilitate anti-tumor immune responses in breast cancer

    NR4A3 potentials M1-like macrophage polarization to facilitate anti-tumor immune responses in breast cancer

    Tissue collection

    Tumor tissues were collected from 32 patients with BC who underwent tumorectomy (Supplementary Table 2). The use of all the patient material was approved by the Ethics Committee of Tongji Hospital, Tongji Medical College,…

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  • FOXO3-enhanced mesenchymal progenitor cells impede aging in monkeys

    FOXO3-enhanced mesenchymal progenitor cells impede aging in monkeys

    “The low implantation efficiency of human stem cells in the host’s aging microenvironment, their short functional residence time, and the potential risk of tumorigenesis together constitute the technical bottlenecks that restrict the…

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  • Dynamicasome—a molecular dynamics-guided and AI-driven pathogenicity prediction catalogue for all genetic mutations

    Dynamicasome—a molecular dynamics-guided and AI-driven pathogenicity prediction catalogue for all genetic mutations

    Data generation workflow

    Using in-house scripts, we created a workflow to date patient gene (accession number) to go from cDNA sequence (NCBI) and generate all missense variants encoded for that cDNA, which in the case of PMM2 is 1454 variants….

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  • FACEDIG automated tool for placing landmarks on facial portraits for geometric morphometrics users

    FACEDIG automated tool for placing landmarks on facial portraits for geometric morphometrics users

    Landmark description

    Applying geometric morphometrics to human facial shape involves the precise use of landmarks to capture facial morphology. Ideally, landmarks should denote key morphological features as discrete, anatomically homologous points…

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  • The clinical application value of the KU-F40 fully automatic fecal analyzer for the detection of fecal parasites: A large-sample retrospective study

    The clinical application value of the KU-F40 fully automatic fecal analyzer for the detection of fecal parasites: A large-sample retrospective study

    Global public health exigency in improving the sensitivity of parasite detection

    Intestinal parasites reside in the human intestine. Among the wide variety of species, more than 300 types of parasites can be transmitted to human5, including but…

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  • Author Correction: Spatial transcriptomics reveals regionally altered gene expression that drives retinal degeneration

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  • Nuclear morphometrics coupled with machine learning identifies dynamic states of senescence across age

    Nuclear morphometrics coupled with machine learning identifies dynamic states of senescence across age

    Animal models

    Animal experiments were in accordance with the Institutional Animal Care and Use Committee (IACUC, protocols 202100001/2) approved by the New York University Grossman School of Medicine. All mice were on a C57BL/6 J background and…

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