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He, F. et al. The global decline of…
Category: 5. Biology
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Non-retroviral RNA viruses in eukaryotic genomes
Non-retroviral RNA viruses have RNA genomes but do not use reverse transcription, and they are the most common type of virus to infect eukaryotes. Such viruses with high epidemiological relevance include Dengue, Chikungunya, West Nile and Zika…
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Head and neck tumor organoid grown under simplified media conditions model tumor biology and chemoradiation responses
Siegel, R. L., Miller, K. D., Wagle, N. S. & Jemal, A. Cancer statistics, 2023. CA Cancer J. Clin. 73, 17–48 (2023).
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Vatte, C. et al. Tyrosine kinase domain…
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Single-cell transcriptomic analysis identifies systemic immunosuppressive myeloid cells and local monocytes/macrophages as key regulators in polytrauma-induced immune dysregulation
Experimental design and scRNA-seq pipeline
In this study, transcriptomic analysis was performed using a rat polytrauma model of bone nonunion and volumetric muscle loss. The cells from the blood, bone marrow, and the local defect tissue were…
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Global increase in the endemism of birds from north to south
Global patterns of diversity and endemism
Alpha diversity peaks in the tropics and generally declines towards higher latitudes in both hemispheres (Figs. 1, 2a). It thus follows the classic pattern of a latitudinal gradient in diversity43, albeit…
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Structural insights into transcriptional regulation by the helicase RECQL5
Protein purification
GST-tagged RECQL51–620 was expressed in E. coli BL21-CodonPlus (DE3)-RIL cells (Stratagene) for 16 h at 18 °C and the cells were lysed with a cell disrupter (Avestin)22. The clarified lysate was loaded onto a…
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Genomic analysis of Plasmodium vivax field isolates circulating in sub-Saharan Africa
Samples
A total of 133 P. vivax field isolates were obtained between 2016 and 2021 from symptomatic patients originating from ten sub-Saharan African countries. Samples were collected locally at health centers in Ethiopia (N = 66),…
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Feather macrostructure is marginally correlated with temperature range but not urbanization across California
Oke, T. R. City size and the urban heat island. Atmos Environ 7, 769–779 (1973).
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Mokhtari, Z., Barghjelveh, S., Sayahnia, R., Qureshi, S. & Russo, A. Dynamic and…
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RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteria
S. pyogenes culture and mutagenesis
Streptococcus pyogenes strain SF370; M1 GAS and its derivatives were cultured on Trypticase soy agar (BD Difco) plates supplemented with 5% defibrinated sheep blood. In THY, C medium and…
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Neurogenomic and behavioral principles shape freezing dynamics and synergistic performance in Drosophila melanogaster
Ethics
Experiments using transgenic Drosophila melanogaster stocks were approved under Chiba University Institutional Biosafety Committee protocol #G5-46.
Fly strains and keeping conditions
A cohort of 104 strains derived from the DGRP was used in…
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