This study offers a comprehensive examination of the clonal dynamics of invasive pneumococcal isolates in Denmark from the summer of 2019 to the end of 2023. We utilized GPSCs, STs, and serotype distribution data to analyze the impact of…
Category: 5. Biology
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Intersectin and endophilin condensates prime synaptic vesicles for release site replenishment
Animal use
All the animal work was performed according to the National Institutes of Health guidelines for animal research with approval from the Animal Care and Use Committees at the Johns Hopkins University School of Medicine. For Itsn1 KO…
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Exploring the role of inflammation, immunity, and metabolism in colorectal cancer via mendelian randomization approach
MR and sensitivity analysis results
Through MR analysis of 4,907 circulating plasma proteins, 91 inflammatory proteins, 731 immune cell traits, and 1,400 metabolites, we identified a series of biomarkers with causal relationships to CRC. Detailed…
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Identifying climate refugia and bright spots for highly mobile species
Scheffers, B. R. et al. The broad footprint of climate change from genes to biomes to people. Science 354, aaf7671 (2016).
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Bryndum-Buchholz, A. et al. Twenty-first-century climate…
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Selection of stable reference genes for accurate reverse-transcription quantitative PCR in cotton-herbivore studies using virus-induced gene silencing
Taylor, S., Wakem, M., Dijkman, G., Alsarraj, M. & Nguyen, M. A practical approach to RT-qPCR—Publishing data that conform to the MIQE guidelines. Methods 50, S1–S5. https://doi.org/10.1016/j.ymeth.2010.01.005 (2010).
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Anti-IgD nanobodies as novel tools for studying human IgD biology
Plasmids and cloning
Cloning was performed using standard PCR methods with Q5 High-Fidelity 2× Master Mix and NEBuilder HiFi DNA Assembly Cloning Kit (NEB). Coding sequences were verified by Sanger sequencing (Source Bioscience or Eurofins…
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The PARP inhibitor talazoparib synergizes with reovirus to induce cancer killing and tumour control in vivo in mouse models
Cell lines
The following melanoma cell lines of known genetic background were used and obtained from stocks within Prof. Kevin Harrington’s team, ICR London: A375 and Mel624 (V600EBRAF mutant), WM266.4 (V600DBRAF mutant), MeWo and PWMK (wild…
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Evolutionary adaptations of cyanobacterial polyhydroxybutyrate (PHB) biosynthesis and metabolic pathways in Spirulina, Arthrospira, and Limnospira spp
Thajuddin, N. & Subramanian, G. Cyanobacterial biodiversity and potential applications in biotechnology. Curr. Sci. 89, 47–57 (2005).
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Gevin, M., Latifi, A. & Talla, E. The modular…
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R-loops: a key driver of inflammatory responses in cancer
Petermann, E., Lan, L. & Zou, L. Sources, resolution and physiological relevance of R-loops and RNA–DNA hybrids. Nat. Rev. Mol. Cell Biol. 23, 521–540 (2022).
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