General information
All reagents were purchased from commercial suppliers and used without further purification. Detailed information on the reagents and resource is listed in Supplementary Table 3. The fluorescent HaloTag substrates were…
Category: 5. Biology
-
Escape rooms for proteins | Nature Methods
Understanding the molecular properties of diverse biomolecules is a long-standing challenge in biochemistry and biophysics. However, methods for high-precision and high-throughput characterization of molecules in solution are still needed.
Madhavi…
Continue Reading
-
Intermediate filament IFFO1 negatively regulates the migration of lung cancer cells by inhibiting the IQGAP3-Cdc42 interaction
Clinical specimens and tissue microarray
This study was approved by the Ethics Committee of Chongqing Medical University (Number: 2024036) and followed the Declaration of Helsinki. The slices of tumor tissue were taken from the Department of…
Continue Reading
-
Sexual dimorphism in response to intermittent fasting and its impact on the brain
Fontana, L., Partridge, L. & Longo, V. D. Extending healthy life span–from yeast to humans. Science 328 (5976), 321–326 (2010).
Google Scholar
McCay, C. M.,…
Continue Reading
-
A high-performance genetically encoded sensor for cellular imaging of PKC activity in vivo
Ethical statement
All surgical and experimental procedures were performed in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals, written by the National Research Council (US) Institute for Laboratory Animal…
Continue Reading
-
Biochemical, antioxidant activity, and phenological diversity among Berberis progenies in the F1 population
Phenological characters
This study conducted a thorough evaluation of phenological traits, including the onset of flowering, full bloom, petal abscission, and fruit harvesting time, across a diverse collection of 91 barberry genotypes, as…
Continue Reading
-
Colon cancer cells evade drug action by enhancing drug metabolism
Glucuronidation pathway is required for trametinib resistance in RAP tumours
The Drosophila hindgut has proven a useful tool for modelling CRC including for predicting therapeutics [4]. To identify the most effective inhibitor against RasG12V…
Continue Reading
-
Structural and functional characterization of a porcine intestinal microbial ecosystem developed in vitro
Significant differences in microbial composition between the in vivo and SPIME communities
Shallow shotgun sequencing was used to detail the microbial composition and structure of the in vivo samples obtained from the four pigs’ colons, feces,…
Continue Reading
-
Sequencing DNA in the air
Besides being used to routinely sequence various biological samples, DNA sequencing has now been applied to environmental samples that contain trace amounts of DNA that are shed from animals, plants and microorganisms. Such environmental DNA…
Continue Reading
-
Study on the spatial and temporal differentiation of intangible cultural heritage and its influencing factors in Shandong province
Analysis of spatial distribution characteristics
Types of overall spatial differentiation characteristics
-
(1)
Geographic Concentration Index Analysis
…
Continue Reading
-
(1)