Generation of inducible MSR-dCas9-effector components
To generate inducible dCas9-effector components the following plasmids were obtained from Addgene: pB-TRE-dCas9-VPR (63800), pB-CAGGS-dCas9-KRAB-MeCP2 (110824), pB-CAGGS-dCas9 (118023) and…
Category: 5. Biology
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Author Correction: The importance of physiological and disease contexts in capturing mRNA modifications
Author Correction: The importance of physiological and disease contexts in capturing mRNA modifications
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Substrate binding and coupled mechanisms of Vps4p substrate recruitment and release from autoinhibition
Peptide synthesis
All peptides were synthesized using solid-phase synthesis, Fmoc chemistry, and a C-terminal amide group. Unlabeled peptides were N-terminally acetylated. For peptides used in fluorescence polarization assays,…
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Treatment of non-small cell lung cancer using chem-bioinformatics-driven engineering of exosomal cargo-vehicle for telmisartan and pioglitazone targeted-delivery
Bioinformatics studies
This study is part of a bigger project in our group that uses natural products that have been changed into new uses, like prodigiosin, camptothecin, and hinokitiol, to target different types of cancer49,50,51. The project…
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NFATc1 deficiency in B cells ameliorates atopic dermatitis
Mice
6–8 weeks old C57BL/6 Nfatc1f/f x mb1cre mice and wild type mice were used for AD induction. Nfatc1f/f x mb1cre mice line was generously provided by Dr. Edgar Serfling (Department of Pathology, University of Wuerzburg, Germany). Mice were…
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A telomere-to-telomere gap-free genome assembly of the endangered humphead wrasse (Cheilinus undulatus)
Sample collection
We obtained an adult humphead wrasse (Fig. 1a) from Guangdong Marine Fisheries Experimental Centre, an offsite facility of the Agro-Tech Extension Center of Guangdong Province, which is situated in Huizhou city, Guangdong…
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Enhancer mutational screen in vivo
Transcriptional enhancers are key to dynamic gene regulation, but current knowledge on which sequences and motifs are essential for proper enhancer function is still incomplete. This limits our ability to interpret the relevance of enhancer…
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Fortilin deficiency induces anti-atherosclerotic phenotypes in macrophages and protects hypercholesterolemic mice against atherosclerosis
Animal study
We conducted all animal experiments using male mus musculus mice, 8 weeks of age or older, on the C57BL/6 J genetic background, and followed all relevant ethical regulations for animal use. Specifically, qualified vivarium staff…
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A marker for sterol-dependent metastatic tropism
A marker for sterol-dependent metastatic tropism
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