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Category: 5. Biology
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Towards a quality control framework for cerebral cortical organoids
The stochastic nature of the differentiation of stem cells and their spontaneous self-organization within cerebral organoids leads to unpredictable variability among them1. Consequently, novel approaches have recently emerged to improve culture…
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Effect of substrate mineralogy, biofilm and extracellular polymeric substances on bacterially induced carbonate mineralisation investigated with in situ nanoscale ToF-SIMS
Dubey, A. A., Dhami, N. K., Ravi, K. & Mukherjee, A. Erosion mitigation with biocementation: a review on applications, challenges, & future perspectives. Rev. Environ. Sci. Bio/Technology. https://doi.org/10.1007/s11157-023-09674-z (2023).
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Co-profiling of in situ RNA-protein interactions and transcriptome in single cells and tissues
Ethics statement
All animal experiments were approved by Institutional Animal Care and Use Committee (IACUC) of Peking University, which are accredited by the AAALAC (Association for Assessment and Accreditation of Laboratory Animal Care…
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Mechanistic insights into the versatile stoichiometry and biased signaling of the apelin receptor-arrestin complex
APJR-βarr1 complexes with distinct stoichiometry
To promote the assembly of a functional complex, we followed an established protocol for GPCR-βarr1 structural determination24. Briefly, the C-terminal segment (residues C331-D380) of the APJR was…
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A gut instinct for microbial patterns
Growing evidence indicates that colonic microbes can influence feeding behaviour, but a neural circuit capable of transducing microbial sensory signals such as microbe-associated molecular patterns (MAMPs) from the gut to the brain has not yet…
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Guanine is an inhibitor of c-jun terminal kinases
Cell cultures
All procedures were carried out under the UK Animals (Scientific Procedures) Act (1986) and approved by the University of Manchester Review Ethics Panel. PER2::LUC MEFs were prepare from heterozygous PER2::LUC embryos17 at day…
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The insulin signalling network | Nature Metabolism
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