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Category: 5. Biology
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The bile acid-sensitive ion channel is gated by Ca2+-dependent conformational changes in the transmembrane domain
hBASIC adopts a modified ASIC fold
To verify that hBASIC is modulated by Ca2+, we injected Xenopus oocytes with mRNA encoding full-length hBASIC and recorded current change in response to the removal of Ca2+o using two-electrode voltage clamp…
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HIF-1α alleviates ferroptosis in ulcerative colitis by regulation of GPX4
Reagents
The primary antibodies were listed as follows: For Western blot: HIF-1α (1:1000, ab179483, abcam), FTH (1:1000, ab183781, abcam), FTL (1:10000, ab69090, abcam), zonulin (1:1000, ab256454, abcam), ZO-1 (1:1000, ab276131, abcam), Occludin…
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The essential role of gut microbiota in dsRNA-mediated pest control of the phytophagous ladybird beetle, Henosepilachna vigintioctopunctata
Plant and insect materials
Solanum nigrum L. seeds underwent sterilization and vernalization at 4 °C for 24 h before germination on Murashige and Skoog (MS) medium. Seedlings were subsequently transplanted into sterile culture bottles or…
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Structural basis for the activity regulation of Medicago calcium channel CNGC15
Architecture of MtCNGC15b
To explicate the structure of MtCNGC15, the expression level and cryo-EM performance of three isoforms MtCNGC15a/b/c were individually examined using insect cells and mammalian cells. In spite of high sequence…
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Ripk1 is critical for preserving effector regulatory T cells and the suppressive transcriptional program in regulatory T cells
Mice
Ripk1tm1.1Pvdb, B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr/J, Foxp3tm1(CD2/CD52)Shori, and B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J mice have been previously described [56,57,58,59]. Ripk1ΔFoxp3 mice and Ripk1fl/fl Foxp3Cre/wt mice were generated by…
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Specific oncogene activation of the cell of origin in mucosal melanoma
This research complies with all relevant ethical regulations. Appropriate study approvals are included in the relevant sections below.
Zebrafish
Cloning
For the tfap2b-GFP reporter, two open areas of chromatin that were statistically enriched in…
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Footwear has a modifying effect on tibial loading during military weight carriage
Tibial loading metrics
Tibial bending moments
Fig. 1 Mean time-series data of resultant bending moment (A), JRF component of bending moment (B), and muscular force component of bending moment (C) during the stance phase of walking under different…
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Machine learning and microfluidic integration for oocyte quality prediction
The experimental test was carried out for 54 immature oocytes. At first, each oocyte was individually injected into the channel with a very low flow rate of about 0.05 µl per minute. The flow was then increased stepwise at intervals of 10 s….
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