Generation of knockout and miR-E-expressing mES cells
Lamin TKO (vial ‘TKO97’, passage 12) and littermate WT (vial ‘28538’, passage 9) mES cells were from the laboratory of Dr. Yixian Zheng43. Lamin TKO was validated by endpoint PCR using…
Category: 5. Biology
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Listening to forest recovery | Nature Reviews Biodiversity
Tropical forests are among the largest carbon stores on Earth and home to two-thirds of the world’s biodiversity. Yet, these forests face relentless threats that include habitat loss, climate change and human activities such as logging or…
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Combined single-cell profiling of chromatin–transcriptome and splicing across brain cell types, regions and disease state
Ethics statement
All experiments were conducted in accordance with the 2011 Eighth Edition of the NIH Guide for the Care and Use of Laboratory Animals. Animal procedures were performed according to protocols approved by the Animal Care and Use…
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Personalized targeted memory reactivation enhances consolidation of challenging memories via slow wave and spindle dynamics
Participants
A total of 42 healthy participants (16 females, mean age: 26.57 ± 3.13 years) were initially recruited. All participants had normal hearing and vision and were instructed to refrain from caffeine, alcohol, and central nervous…
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Phosphorylation of USP33 by CDK1 stabilizes the mTORC2 component SIN1
Cell culture, plasmids, and antibodies
HEK293T, PANC-1, MIA PaCa-2 cells, and other lines were obtained from American Type Culture Collection (ATCC). HEK293T, PANC-1 cells were cultured in Dulbecco’s modified Eagles’s medium (Gibco)…
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Genome assembly of two allotetraploid cotton germplasms reveals mechanisms of somatic embryogenesis and enables precise genome editing
Bhatia, S., Sharma, K., Dahiya, R. & Bera, T. (eds). Modern Applications of Plant Biotechnology in Pharmaceutical Sciences, pp. 209–230 (Academic Press, 2015).
Zheng, Q. & Perry, S. E. Alterations in the transcriptome of Soybean in response to…
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Hematological and biochemical profiles, infection and habitat quality in an urban rat population
Fenton, A., Knowles, S. C., Petchey, O. L. & Pedersen, A. B. The reliability of observational approaches for detecting interspecific parasite interactions: comparison with experimental results. Int. J. Parasitol. 44, 437–445.
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Multifaceted role of Trichoderma harzianum isolates in mitigating drought stress and promoting adaptive responses in barley cultivars
Boken, V. K., Cracknell, A. P. & Heathcote, R. L. Monitoring and Predicting Agricultural Drought: A Global Study (Oxford University Press, Oxford, 2005).
Sallam, A., Alqudah, A. M., Dawood, M….
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Heatwaves disrupt prey behaviour | Nature Climate Change
Calvin, K. et al. in Climate Change 2023: Synthesis Report (eds Core Writing Team, Lee, H. & Romero, J.) Section 2, 42–43 (IPCC, 2023).
Frich, P. et al. Clim. Res. 19, 193–212 (2002).
Walsh,…
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Bioinformatic exploration reveals features of tenpIN family of type III toxin-antitoxin systems in bacteria and viruses
TenpIN TA systems are widespread in the microbial world
The initial bioinformatics study conducted over a decade ago identified 25 putative11 tenpIN sequences, of which 11 were well-annotated and remain in current…
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