Category: 5. Biology

Genome re-sequencing and comparison between two sequencing methods

We successfully extracted DNA from 258 adult female individuals collected from 25 field populations across China (Fig. 1a, Supplementary Data 1–2). Subsequently, we…

Sample collection and DNA sequencing

The C. barosmum sample was collected from Lijiang City, Yunnan Province, China, at coordinates 27°26′00″N, 100°10′43″E. Genomic DNA was successfully extracted from C. barosmum leaves using the…

Cells and plasmids

Dictyostelium discoideum cells were cultured in HL5 media⁹¹ for a maximum of 2 months after thawing from frozen stock. AX3 cells were obtained from the R. Kay laboratory (MRC Laboratory of Molecular Biology, UK), abnABC⁻ cells…

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This is a summary of: Zhou, Y. et al. Cross-species analysis of adult hippocampal neurogenesis reveals…

Medulloblastoma, the most prevalent pediatric brain tumor, is characterized by four distinct molecularly defined consensus groups: WNT-activated, Sonic hedgehog (SHH)-activated, Group 3 (G3), and Group 4 (G4). Each group is marked by distinct…

Force-dependent regulation of DNA replication by SSB proteins

The binding of T7 SSB to ssDNA reportedly depends on force-induced ssDNA conformations¹³, suggesting this interaction may modulate DNA replication in a force-dependent manner. We…

Cell culture

HEK293T (American Type Culture Collection (ATCC), CRL-11268) were cultured as recommended by the ATCC. Cell lines tested negative for Mycoplasma and were authenticated by the suppliers.

Modeling of gene-editing outcomes

The inDelphi…

Animals

Male Sprague Dawley (SD) rats weighing 200–250 g were obtained from Shanghai Bikai Keyi Biotechnology Co., Ltd. and housed at the Animal Center of Naval Medical University under specific pathogen-free conditions. Care, breeding, and…