Production of N protein antigen
The full-length N gene of MERS-CoV was synthesized and cloned into the pET28a vector harboring an N-terminal 6×His tag. Then, the N-terminal domain (NTD, 1-492 nucleotide) and C-terminal domain (CTD, 502–1239…
Category: 5. Biology
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Vasoprotective effects of lysophosphatidic acid inhibit vascular injury caused by SARS-CoV-2 infection
Viruses
Various SARS-CoV-2 strains, hCoV-19/Japan/QHN002/2021 (Alpha), hCoV-19/Japan/TY8-612/2021 (Beta), hCoV-19/Japan/TY7-503/2021 (Gamma), hCoV-19/Japan/TY11-927/2021 (Delta), and 2019-nCoV/Japan/TY38-873/2021 (Omicron), isolated at the…
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Polymerase spiral reaction assay for rapid visual detection of Mycobacterium avium subsp. paratuberculosis in fecal samples
Ethical permission
The study was approved with approval no. IAEC/18/25 by Institutional Animal Ethics Committee (IAEC), Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura,…
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The RNA-binding protein RRP1 brakes macrophage one-carbon metabolism to suppress autoinflammation
Ethics statement
The study design and experimental procedure for peripheral blood samples collected from patients were approved by the Ethics Review Board of Peking Union Medical College Hospital and Institute of Basic Medicine, Chinese Academy of…
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Viral hepatitis: breaking down barriers
With just 5 years to go until the WHO’s goal to eliminate viral hepatitis as a public health threat by 2030, focus must turn to the inequitable access to testing and treatment across the world, and to breaking down the barriers that prevent…
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Efficacy and safety of IBI351 (fulzerasib) monotherapy in KRASG12C inhibitor-naïve Chinese patients with KRASG12C-mutated metastatic colorectal cancer: a pooled analysis from phase I part of two studies
KRAS was historically regarded as an “undruggable” target until the development of KRASG12C allele-specific inhibitors. The discovery of the switch II pocket within the KRAS protein has rapidly accelerated the development of KRASG12C…
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Toxic effects of Helix aspersa snail egg hydrolyzates obtained by static in vitro digestion on Caco-2 colorectal adenocarcinoma cells
High cell content of reactive oxygen species (ROS) could result in oxidative stress, and antioxidants prevent their formation and inhibit their activity2. Certain antioxidants could have beneficial effects on colorectal cancer initiation and…
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Pericyte-mediated regulation of angiogenesis during cutaneous wound healing in adult zebrafish
Conditional ablation of mural cells in zebrafish larvae
To clarify the roles of pericytes in regulating angiogenesis during wound healing, we generated a transgenic zebrafish line that enables the conditional ablation of mural cells by employing…
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The role of glucocorticoids in hepatocellular carcinoma through integrated bioinformatics analysis and experimental validation
Tumor and control group differential expression analysis and GSEA enrichment analysis
Through comparing tumor and control groups, 751 DEGs were identified, showing a statistically significant difference between the two groups (|(log2FC)| >1.5, P…
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