Cell culture
Human lung adenocarcinoma A549 cells (American Type Culture Collection (ATCC) CCL-185, gift from the lab of P. Hofman, IRCAN, Nice, France), human breast adenocarcinoma MDA-MB-231 cells (gift from the lab of D. Picard, University of…
Category: 5. Biology
-
YAP charge patterning mediates signal integration through transcriptional co-condensates
mESC culture maintenance and spontaneous differentiation
E14 mESCs (gift from the Panning lab, UCSF) were maintained on gelatin coated dishes in 2i+LIF media, composed of a 1:1 mixture of DMEM/F12 (Thermo Fisher, 11320-033) and Neurobasal (Thermo…
Continue Reading
-
Tracking HPAIV H5 through a geographic survey of Antarctic seabird populations
The first-time detection of suspected cases of HPAIV H5 influenza in penguins and cormorants in the Antarctic continent could mark a significant expansion of the panzootic into this isolated continent and puts highly vulnerable bird populations…
Continue Reading
-
Phased genome assemblies and pangenome graphs of human populations of Japan and Saudi Arabia
Sample processing and sequencing
Saudi samples
Fresh blood was collected from the donors and shipped to King Abdullah University of Science and Technology (KAUST) (Thuwal, Saudi Arabia) in ambient temperature for DNA extraction. The donors were…
Continue Reading
-
Transcriptome analysis reveals that regulation network of the genes related to unique double flowers in tropical viviparous water lily
Phenotypic characterization of double flower in N. ‘Candy Rain’
N. ‘Candy Rain’ represents a unique tropical viviparous water lily cultivar characterized by its distinctive double-flower morphology. The floral architecture displays three…
Continue Reading
-
Structural basis for membrane microdomain formation by a human Stomatin complex
Lande, W. M., Thiemann, P. V. & Mentzer, W. C. Jr. Missing band 7 membrane protein in two patients with high Na, low K erythrocytes. J. Clin. Invest 70, 1273–1280 (1982).
Google Scholar
…Continue Reading
-
How the American Cancer Society is tackling the collaboration challenge in cancer research
REGISTER NOW
Collaboration is key to advancing cancer research, but institutional silos, fragmented data, and complex workflows often slow progress. Researchers need secure, scalable environments that enable seamless data sharing…
Continue Reading
-
Glutamatergic synaptic resilience to overexpressed human alpha-synuclein
Primary hippocampal neuronal cultures from rats
Preparation of primary hippocampal neuronal cultures from E18 Wistar rat embryos was carried out as previously described with slight modifications65,66. All animal procedures were performed in…
Continue Reading
-
A citrullinated histone H3 monoclonal antibody for immune modulation in sepsis
A thematic approach to achieve optimization of the humanized CitH3 monoclonal antibody
To create the humanized hCitH3-mAb from the original mouse antibody40, a multi-step approach was used to maintain binding specificity while reducing…
Continue Reading
-
Recreating fibroblast diversity in vitro by activating transcription factors
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Southard, K. M. et al. Comprehensive transcription factor perturbations recapitulate…
Continue Reading