Reagents
The following reagents were used: BAPTA-AM (196419), diphenyleneiodonium chloride (DPI) (300260), Calbiochem; phorbol 12-myristate 13-acetate (PMA) (P8139), thapsigargin (T9033), LPS E.coli (L2630), homovanillic acid (306-08-1),…
Category: 5. Biology
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Comparing the precision of two digital PCR applications for copy number comparisons in protists
Hutchins, D. A. & Fu, F. Microorganisms and ocean global change. Nat. Microbiol. 2, 1–11 (2017).
Tilman, D. et al. The influence of functional diversity and composition on ecosystem…
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Spatially constrained disulfide bond shuffling delays insulin aggregation and enhances neurotoxicity
Spatial distance-confined Insulin DBS
We previously have demonstrated the distance confinement for bicarbonate heating-induced DBS products likely ranges from 2 Å (the length for disulfide bond) to over 9 Å21. For insulin, the crystal…
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Contribution of phenolic compounds and hormones in antioxidant defense responses of wild and cultivated barley genotypes under drought and heat stress
The influence of environmental conditions on traits
The results of the combined analysis of variance (ANOVA) are summarized in Table 1. Significant effects were observed for genotype, irrigation regime (drought), planting date (heat), and their…
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Single nucleus RNA sequencing profile analysis to reveal cell type specific common molecular drivers of Parkinson’s disease and therapeutic agents
Reeve, A., Simcox, E. & Turnbull, D. Ageing and parkinson’s disease: why is advancing age the biggest risk factor? Ageing Res. Rev. 14, 19–30 (2014).
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Curcumin exerts neuroprotective effects on proliferation of neural stem cells in vitro and APP/PS1 mouse model in vivo
Primary NSC culture and expansion
Neural stem cells (NSCs) were cultured to generate neurospheres according to the protocols established by Reynolds and Weiss39. Telencephalic tissue was isolated from embryonic day 16 (E16) mice and subjected to…
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Targeting Langerhans cells via skin delivery of HIV Envelope enhances the antibody response to vaccination
Vaccine constructs and formulations
The αLang.Env vaccine constructs have been designed and produced as previously published34. Briefly, the anti-Langerin mAb was developed in-house (clone 4C731, GenBank sequences JX002668, JX002669) and…
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Octenidine effectively reduces Candida auris colonisation on human skin
Ingham, C. J. et al. Rapid susceptibility testing and microcolony analysis of Candida spp. cultured and imaged on porous aluminum oxide. PLoS ONE 7, 33818. https://doi.org/10.1371/journal.pone.0033818 (2012).
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Decoding cell fate: human models reveal how SMAD2 variants shape development
The ability to sequence genomes at scale has revealed a vast landscape of rare and de novo variants in patients with congenital heart disease (CHD) and other complex disorders. Yet how do we decode the functional impact of these variants,…
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