Category: 5. Biology

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This is a summary of: Liu, Y. et al. LARP4-mediated hypertranslation drives T cell dysfunction in tumors….

This research does not involve human participants, animal subjects, or the use of biological samples requiring institutional ethics approval. All relevant ethical regulations were followed.

Overview of cpDistiller

cpDistiller consists of three main…

Patients

Paraffin-embedded samples of 50 primary DLBCL patients and 30 reactive hyperplasia patients in Shandong Provincial Hospital between 2014 and 2019 were collected. Samples of reactive hyperplasia were used as controls. Peripheral blood,…

Cloning and protein purification

GATOR2 purification

Codon-optimized DNA encoding all five subunits of GATOR2 (MIOS, WDR59, WDR24, SEH1L and Sec13) was synthesized by Twist Biosciences and subcloned into the pCAG vector. The construct with MIOS was…

Engineering patterned ECM substrates of defined area

To create defined areas of ECM proteins, we used μCP to print arrays of circular collagen I patterns to which HeLa (Kyoto) cells can adhere via α1β1 and α2β1 integrins⁵⁷ and circular…

Production of N protein antigen

The full-length N gene of MERS-CoV was synthesized and cloned into the pET28a vector harboring an N-terminal 6×His tag. Then, the N-terminal domain (NTD, 1-492 nucleotide) and C-terminal domain (CTD, 502–1239…

Viruses

Various SARS-CoV-2 strains, hCoV-19/Japan/QHN002/2021 (Alpha), hCoV-19/Japan/TY8-612/2021 (Beta), hCoV-19/Japan/TY7-503/2021 (Gamma), hCoV-19/Japan/TY11-927/2021 (Delta), and 2019-nCoV/Japan/TY38-873/2021 (Omicron), isolated at the…

Ethical permission

The study was approved with approval no. IAEC/18/25 by Institutional Animal Ethics Committee (IAEC), Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura,…

Ethics statement

The study design and experimental procedure for peripheral blood samples collected from patients were approved by the Ethics Review Board of Peking Union Medical College Hospital and Institute of Basic Medicine, Chinese Academy of…