The mycorrhizal colonization rate was found to be significantly influenced by fungi and herbicides (P ≤ 0.01). However, the application methods of the herbicides were not effective in the greenhouse experiment. In plants that were not…
Category: 5. Biology
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Identification and validation of biomarkers associated with glycolysis in polycystic ovarian syndrome
Boyle, J. A. & Teede, H. J. PCOS: refining diagnostic features in PCOS to optimize health outcomes. Nat. Reviews Endocrinol. 12 (11), 630–631 (2016).
Li, J. et al. Effect of exposure to…
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A complex between IF2 and NusA suggests early coupling of transcription-translation
Labeling of IFs and fMet-tRNAfMet
To fluorescence label the IFs for live-cell single-molecule tracking, we explored if the addition of a HaloTag compromises IF activity in supporting cell growth (Supplementary Note 1)….
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Longitudinal transcriptomic and epigenetic analysis of the blood in two astronauts
Whole blood transcriptomics
Two participants provided three blood samples each (Fig. 1A). The blood samples were collected at the pre-flight mark (T0, within two weeks before departure), at post-flight (T2, within 24 hours after landing), and at…
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Removal of E. coli O157:H7 coliform bacteria from sewage wastewater using silver doped borate bioglass
Preparation of BAgX BG samples
Five types of silver-doped borate BG samples, BAg0, BAg1, BAg2, BAg3, and BAg4, were prepared by the melt-quenching method at high temperatures between 1100 and 1200 °C as spherical-shaped discs (Fig. 2). After the…
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A genetically encoded biosensor for point-of-care and live-cell detection of d-2-hydroxyglutarate
Ethical statement
All procedures were performed in compliance with relevant laws and institutional guidelines. Animal studies were approved by the Second Hospital of Shandong University Ethics Committees (Approval No. KYLL-2022A128). Human…
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In vivo efficacy of NRL knockdown with cell-penetrating siRNA in retinal degeneration
Design and synthesis of cp-asiNRL
Sequences targeting NRL were designed for homology with Homo sapiens, Rattus norvegicus, and Mus musculus. Sequence information is provided in Supplementary Table 1. Knockdown efficacies were validated in HeLa…
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Effects of abiotic and biotic elicitors on tomato resistance to tomato brown rugose fruit virus
Sources and Preparation of bacterial and viral isolates
The bacterium P. fluorescens 1442 (P) was obtained from the bacterial collection at the Agricultural Sciences and Natural Resources University of Khuzestan (Mollasani, Iran), and the…
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Pediatric gastrointestinal basidiobolomycosis: descriptive bicenteric retrospective study
Gastrointestinal basidiobolomycosis (GIB) is a rare but increasingly recognized fungal infection, particularly among pediatric patients in tropical and subtropical regions. The first documented pediatric case was reported in 1964 in a 6-year-old…
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Design of a multi-epitope vaccine against drug-resistant mycobacterium tuberculosis and mycobacterium bovis using reverse vaccinology
Study design
An in silico approach depicted in Figure S2 and utilizes bioinformatics computational methods, specifically reverse vaccinology and molecular docking techniques, was used in this study. Figure 1 represents the methodology’s…
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