Category: 5. Biology

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  • Evolutionary trajectory and spread of respiratory syncytial virus group A in neonatal cohorts in Pakistan amidst the COVID-19 pandemic

    Evolutionary trajectory and spread of respiratory syncytial virus group A in neonatal cohorts in Pakistan amidst the COVID-19 pandemic

    RT-PCR positivity and RSV sequence diversity in the cohort:

    From 2019 to 2022, 758 paediatric patients ages 0–24 months in six Pakistani hospitals were tested for RSV and Influenza (Fig. 1). Starting in September 2021, admitted patients were…

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  • Identification of a key smooth muscle cell subset driving ischemic cardiomyopathy progression through single-cell RNA sequencing

    Identification of a key smooth muscle cell subset driving ischemic cardiomyopathy progression through single-cell RNA sequencing

    Overview of the study

    Cardiomyopathy is strongly associated with ferroptosis. The scRNA-seq of 4,184 SMCs found that C6 S100A4 + SMCs were critical, exhibiting high expression of ferroptosis, oxidative stress, and inflammation, which we…

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  • Removal of promoter CpG methylation by epigenome editing reverses HBG silencing

    Removal of promoter CpG methylation by epigenome editing reverses HBG silencing

    HUDEP2 cell culture

    Human umbilical cord blood derived 2 (HUDEP2) cells were grown in serum free expansion medium (SFEM, StemSpan #9650) supplemented with 50 ng/mL stem cell factor (R&D Systems, #255-SC-200/CF), 3 IU/mL erythropoietin…

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  • Dietary lipids induce PPARd and BCL6 to repress macrophage IL-23 induction after intestinal injury and LPS exposure

    Dietary lipids induce PPARd and BCL6 to repress macrophage IL-23 induction after intestinal injury and LPS exposure

    Intestinal IL-23 and IL-22 reparative responses are lost in HFD-fed DSS-treated mice

    To assess the influence of HFD feeding on antimicrobial responses after intestinal damage, we used our previous model of feeding male C57BL/6 mice with a 10%…

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  • IL-33-primed NLRP3 inflammasome in basophils drives IL-1β production and initiates atopic dermatitis inflammation

    IL-33-primed NLRP3 inflammasome in basophils drives IL-1β production and initiates atopic dermatitis inflammation

    Animals and OXA-induced AD model

    C57BL/6 J WT mice were purchased from SLC Inc. (Shizuoka, Japan). Nlrp3–/–, Asc–/–, Casp1/11–/–, Il1b–/–, and Gsdmd–/– mice were kindly provided by Dr. V. M. Dixit (Genentech, San Francisco,…

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  • Long-term trends in human fatalities from human–elephant conflict in Chhattisgarh, India

    Long-term trends in human fatalities from human–elephant conflict in Chhattisgarh, India

    In Chhattisgarh, various factors influence the overall pattern of HEC, particularly leading to incidents of human fatalities and injuries. Developmental activities including infrastructure projects, agricultural expansions, deforestation have…

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