Synaptic connectomics: status and prospects
Category: 5. Biology
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Synaptic connectomics: status and prospects
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Structural insights into SSNA1 self-assembly and its microtubule binding for centriole maintenance
C. elegans SSNA-1 is required for embryonic cell division
To conduct an in-depth structural and functional study of SSNA1 during development of a multicellular organism, we used C. elegans as a genetically tractable…
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Clinical metagenomics for diagnosis and surveillance of viral pathogens
Smyrlaki, I. et al. Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR. Nat. Commun. 11, 4812 (2020).
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Bayart, J.-L….
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So close yet so far apart: distinct flanking sequence recognition by DNMT3A and DNMT3B
To comprehensively evaluate the sequence selectivity of DNMT3 enzymes, we constructed eight enzyme–DNA complex models based on the available crystal structures: DNMT3A bound to CGC, CGT, CGA, CGG, and DNMT3B bound to CGG, CGT, CGA, CGC. In…
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Expanding the cytokine receptor alphabet reprograms T cells into diverse states
Mice
Female Thy1.2+ C57BL/6 (C57BL/6J) mice (aged 5–6 weeks) and female and male NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice (aged 5–7 weeks) were purchased from Jackson Laboratory. TCR-transgenic Thy1.1+ Pmel-1 mice (B6.Cg-Thy1a/Cy…
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Substrate stiffness regulates triple-negative breast cancer signaling through CXCR4 receptor dynamics
Cell culture
We purchased SUM149 cells from the ATCC (Manassas, VA) and cultured cells in F-12 media supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin (Pen/Strep) (Thermo Fisher Scientific, Waltham, MA), 1% GlutaMAX…
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A transformative preclinical model of rheumatic heart disease
Rheumatic heart disease (RHD) remains the leading cause of acquired heart disease in individuals aged >25 years. Despite being preventable, this condition affects >40 million people worldwide, particularly in low-income countries. RHD is…
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Protein hydrolysates derived from residual after polysaccharide extraction of Chlorella vulgaris biomass improves yield and quality of baby leaf lettuce
Recovery of post aqueous extraction of Chlorella vulgaris residual biomass after polysaccharide extraction
The microalga used in this work, Chlorella vulgaris f. viridis Chodat (strain SAG 211 − 12), belongs to the Division Chlorophyta and…
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Turning back the clock on age-related neurodegeneration
Turning back the clock on age-related neurodegeneration
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The complosome – a key regulator of hematopoietic stem cells and innate immune responses
The discovery of complement by Jules Bordet and Paul Ehrlich dates back over 100 years. It has been described as a system of liver-derived serum-circulating proteins that complement antibody-mediated immune responses [1]. This system comprises…
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