Throughout the thermal polymerization process, thiourea undergoes a phase transition from solid to liquid within the temperature range of 150–160 °C, concomitant with the formation of thioamide functional groups and the release of ammonia…
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Structural analyses define the molecular basis of clusterin chaperone function
Plasmids
The pB-T-PAF-Clu plasmid used for WT Clu expression and purification was constructed by amplifying the coding region of pB-T-PAF-CluStrep22 by PCR. The PCR product was then digested with NheI and NotI and subcloned into the pB-T-PAF…
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Design of an integral sliding mode controller for reducing CO2 emissions in the transport sector to control global warming
Stability analysis examines how the solutions of a system behave as time progresses, particularly in response to changes in initial conditions or parameters. Following are the key steps involved in stability analysis of system Eq. (5).
Local…
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Nanoscale spatiotemporal cluster analysis of expressed and endogenous proteins
Gormal, R. S. & Meunier, F. A. Nanoscale organization of the pre-synapse: tracking the neurotransmitter release machinery. Curr. Opin. Neurobiol. 75, 102576 (2022).
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Income and education show distinct links to health and happiness in daily life
Adler, N. E. et al. Socioeconomic status and health: the challenge of the gradient. Am. Psychol. 49, 15–24 (1994).
Borghesi, S. & Vercelli, A. Happiness and health: two paradoxes. J. Econ….
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Effect on quality and safety of hydrophilic reducing compounds integration into taralli via wine enrichment
Wine and vine shoots
White wine with an ethanol content of 11% (v/v) was purchased in a local retail store. It was obtained from the Malvasia Bianca variety grown in Laterza (Puglia, Italy). The wine had the following chemical characteristics: pH…
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Breast cancer is detectable from peripheral blood using machine learning over T cell receptor repertoires
The study investigated the relationship between T-cell receptor (TCR) repertoires in peripheral blood and tumor status by comparing blood samples from Breast Cancer (BC) patients and Healthy Donors (HD). Leveraging machine learning techniques, a…
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Combining single-cell and bulk RNA sequencing to identify CAF-related signature for prognostic prediction and treatment response in patients with melanoma
Identification of CAF marker genes in the melanoma
To identify melanoma cell types, we obtained public scRNA sequencing data from the GEO dataset GSE115978. We obtained 3,630 high-quality single-cell data after quality control processing and then…
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How animal paw pads got their toughness
Nature, Published online: 08 August 2025; doi:10.1038/d41586-025-02474-1
In creatures that walk on land, a protein called Slurp1 protects skin cells from stress.
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