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  • Genome-wide CRISPR screen for human factors involved in alternative polyadenylation based on differential localization of CD47

    Genome-wide CRISPR screen for human factors involved in alternative polyadenylation based on differential localization of CD47

    CD47 mRNA 3′ UTR length affects its protein localization

    Previous studies have demonstrated that global alterations in mRNA polyadenylation, resulting from the utilization of different PASs, play a crucial role in post-transcriptional gene…

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  • Explainable ML modeling of saltwater intrusion control with underground barriers in coastal sloping aquifers

    Explainable ML modeling of saltwater intrusion control with underground barriers in coastal sloping aquifers

    Figure 1 outlines the methodology for predicting L/La in coastal sloping aquifers with underground barriers using Bayesian-optimized gradient boosting models. A dataset of 456 numerical samples, containing key hydraulic parameters as inputs and…

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  • Self-splicing RNA circularization facilitated by intact group I and II introns

    Self-splicing RNA circularization facilitated by intact group I and II introns

    PIE can begin with the second step of the transesterification process

    Group I introns, which are self-splicing ribozymes, enable RNA splicing without protein assistance, functioning through a two-step transesterification reaction that requires…

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  • F-actin disassembly by the oxidoreductase MICAL1 promotes mechano-dependent VWF-GPIbα interaction in platelets

    F-actin disassembly by the oxidoreductase MICAL1 promotes mechano-dependent VWF-GPIbα interaction in platelets

    A list of reagents and antibodies can be found in Supplementary Data 1.

    Animals

    This project was approved by the local ethical committee CEEA26 and the French government under the number APAFIS#25086-2020032312267714. Mical1fl/fl mice were kindly…

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  • Multimodal machine learning for risk-stratified bundled payments in spinal surgery

    Multimodal machine learning for risk-stratified bundled payments in spinal surgery

    Spine surgery is a major contributor to healthcare costs in the United States1, with its high volume and complexity contributing to an annual economic burden exceeding $24.3 billion2,3. Despite the introduction of VBC frameworks such as BPCI and…

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  • AI enhanced model predictive control for optimizing LPG recovery through integrated computational modeling design of experiments and multivariate regression

    AI enhanced model predictive control for optimizing LPG recovery through integrated computational modeling design of experiments and multivariate regression

    Steady-state process simulation

    The steady-state simulation of the debutanizer unit, as illustrated in Fig. 5, established a baseline for assessing operational performance. Key process variables, including temperature, pressure, and composition…

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