Cell lines, culture conditions, transfections, and infections
Human TNBC cell lines MDA-MB-231, MDA-MB-436, and 4T1 mouse breast cancer cells were purchased from American Type Culture Collection (ATCC). All the cell lines were grown in high…
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Preclinical extracellular matrix-based treatment strategies for myocardial infarction: a systematic review and meta-analysis
Tsao, C. W. et al. Heart Disease and Stroke Statistics—2022 Update: A Report From the American Heart Association. Circulation 145, e153–e639 (2022).
McDonagh, T. A. et al. 2021 ESC Guidelines for the diagnosis and treatment of acute and…
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Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2
Mammalian expression constructs
GCase wt was expressed using a commercially available plasmid (pCMV3-GBA-wt, Sino Biological Inc., Peking, China, #HG12038-UT). The expression construct for the secreted LIMP-2 ectodomain (sLIMP-2) was described…
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Independent evolution of satellite DNA sequences in homologous sex chromosomes of Neotropical armored catfish (Harttia)
White, M. The origin and evolution of multiple sex chromosome mechanisms. J. Genet. 40, 303–306 (1940).
Kitano, J. & Peichel, C. L. Turnover of sex chromosomes and speciation in fishes. Environ. Biol. Fishes 94, 549–558 (2012).
…
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Superior outcomes of haploidentical hematopoietic cell transplantation over chemotherapy in AML patients 55 years or older
Klepin HD, Rao AV, Pardee TS. Acute myeloid leukemia and myelodysplastic syndromes in older adults. J Clin Oncol. 2014;32:2541–52. https://doi.org/10.1200/JCO.2014.55.1564.
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Ovarian vascular aging: a hidden driver of mid-age female fertility decline
Khan, S.S., Singer, B.D. & Vaughan, D.E. Molecular and physiological manifestations and measurement of aging in humans. Aging Cell 16, 624–633 (2017).
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Catching the tide at the flood or being in the right place at the right time
Catching the tide at the flood or being in the right place at the right time
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Deep learning-based mobile application for efficient eyelid tumor recognition in clinical images
We developed and validated a smartphone-based application that provides a holistic and quantitative technique for detecting and identifying eyelid tumors in patients. Our system achieved an AUC of 0.917 on external datasets and proved reliable…
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Stepwise recruitment of chaperone Hsc70 by DNAJB1 produces ordered arrays primed for bursts of amyloid fibril disassembly
Protein purification
αSyn was expressed in DE3 Star E. coli cell lines (Thermo Fisher Scientific). Cells were grown in LB media at 37 °C, 180 rpm until reaching an optical density at 600 nm of 0.6–0.8. Protein expression was induced by…
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Mapping the current landscape of locoregional therapy de-escalation trials in early breast cancer: a systematic review
The search identified 3991 results. After removal of duplicates, 3142 studies were screened for inclusion of which 129 were reviewed in full. 32 were excluded following discussion (Supplementary Table 1). A total of 97 studies (48 (49.5%) RCTs9,
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