Gene expression pattern in ulp2Δ cells is altered after multiple generations
We recently reported that growth and cell-cycle defects in ulp2Δ cells were restored after ~500 generations (G) of continuous culture with repeated cycles of dilution…
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VIRMA promotes NSCLC progression by modifying ADAR m6A and increasing the activity of the TGF-β signaling pathway
Clinical samples and information
NSCLC samples preserved in formalin and embedded in paraffin were obtained from the Second People’s Hospital of Changzhou City. All patients provided informed consent to join the study following the guidelines of…
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Monitoring bands during the Norwegian national day parade: a case study on urban distributed acoustic sensing
In this section the key methodological elements used to analyze the DAS data containing the May 17 parade are described. First, vertical forcing of pedestrians is analyzed via the frequency characteristics in the DAS data. Then, the walking pace…
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Age-associated reduction in ER-Mitochondrial contacts impairs mitochondrial lipid metabolism and autophagosome formation in the heart
Lopez-Otin C, Blasco MA, Partridge L, Serrano M, Kroemer G. Hallmarks of aging: An expanding universe. Cell. 2023;186:243–78.
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Mutlu AS, Duffy J, Wang MC….
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Targeting CD38 immunometabolic checkpoint improves metabolic fitness and cognition in a mouse model of Alzheimer’s disease
Human observational study
The human observational study was approved by the Galilee Medical Center Review Board (IRB approval No. 2019-11735) and the Weizmann Institutional Review Board (IRB approval No. 1595-1). Written informed consent was…
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Gut microbiota modulate immune responses to orally and parenterally administered rotavirus in mice
Virus infection
For acute in vivo RV infection, mice were gavaged with 100 μl 1.33% sodium bicarbonate (Sigma S5761), followed by oral inoculation of 104–105 SD50 or 10 SD50 (to mimic the scenario of oral RV vaccine non-responders) of RV, EC…
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A dynamically phase-adaptive regulating hydrogel promotes ultrafast anti-fibrotic wound healing
Preparation and characterization
Cationic antimicrobial εPL with abundant amide groups along polypeptide chains was reacted with HA-CHO polymer via reversible Schiff base reaction to form F/R gel’s network, in which crystalline CeOv nanozyme…
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A unique spontaneously immortalised cell line from pig with enhanced adipogenic capacity
Cell culture
MSC populations were derived in a previous study from subcutaneous adipose tissue samples of 5 Large White x Landrace male piglets (21 days old or younger), as described53. MSCs were grown in DMEM High Glucose (Gibco, 41965039)…
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A SpA+LukAB vaccine targeting Staphylococcus aureus evasion factors restricts infection in two minipig infection models
Antigen production
Protein vaccine antigens were produced recombinantly in E. coli at Genscript. The lukA and lukB genes for LukAB CC8Δ10C and LukAB RARPR-33 were cloned in a single expression vector behind a T7 promoter, and a non-cleavable…
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Foundation model-driven multimodal prognostic prediction in patients undergoing primary surgery for high-grade serous ovarian cancer
Study population
This retrospective study have been performed in accordance with the Declaration of Helsinki. Ethical approval was obtained from the ethics committee of the First People’s Hospital of Yunnan Province, the ethics committee of…
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