Patient inclusion
From 2019 to 2023, ovarian cancer patients from the Netherlands were enroled under a Western IRB-approved protocol into the TUMOVCA study (Medical Ethics Committee Leiden The Hague Delft, protocol number IRB P18.032). The IRB…
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Pooled CRISPR screens identifies key regulators of bovine stem cell expansion for cultured meat
Building a bovine-specific CRISPR screening system: MSC isolation, validation, transduction, and CRISPR activity assessment
To enhance the proliferation rate of bMSCs for cultured meat production, we performed a CRISPR knockout screen to identify…
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PYM1 limits non-canonical Exon Junction Complex occupancy in a gene architecture dependent manner to tune mRNA expression
Singh, G., Pratt, G., Yeo, G. W. & Moore, M. J. The clothes make the mRNA: past and present trends in mRNP fashion. Annu. Rev. Biochem. 84, 325–354 (2015).
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In-cell proximity target validation methods for heterobifunctional molecules with CRBN- or VHL-binder using AirID
Comparison of interactomes of BRD binder-dependent PROTACs with different E3 binders
The bromodomain extra-terminal (BET) family proteins, including BRD4 and BRD3, are promising anticancer drug targets27,28. ARV-825 and MZ1 were developed to…
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Comparing thermographic heat signatures with joint inflammation detected by ultrasound at the knees in patients with rheumatoid arthritis
A total of 570 knee (3 aspects per knee) thermograms were obtained while 190 knees (bilaterally) were scanned by ultrasound among 95 RA patients. The patient baseline characteristics and demographic data were as follows: mean (SD) age was 56.7…
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Evidence that mitochondria in macrophages are destroyed by microautophagy
Plasmids and cells
Plasmids expressing the fluorescently tagged proteins ALIX, TSG101, CHMP2A, VPS4A, and VPS4B were described previously39. Plasmids expressing the doxycycline inducible E228Q-VPS4A-HA, mCherry-Tomm20-N10, and pCHAC-mt-mKeima were…
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Application of a comprehensive approach to pathogen screening in a stowaway rat on an airplane
Sample collection, dissection and species identification
All methods were carried out in accordance with relevant national and international guidelines and regulations. The removal of the stowaway rat from the airplane follows the regulations…
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Risk factors associated with the development of necrotizing enterocolitis in preterm infants on an exclusive human milk diet: a single-center case-control study
Alsaied A, Islam N, Thalib L. Global incidence of Necrotizing Enterocolitis: a systematic review and Meta-analysis. BMC Pediatr. 2020;20:344.
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Caplan MS, Underwood…
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Uneven participation of independent and contract smallholders in certified palm oil mill markets in Indonesia
Study system
In Indonesia, smallholders often have no control over their status as “independent” or “contract” farmers. This is because oil palm development has been driven by large-scale plantation companies that have typically been…
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