NAI antibody measurements are substrate-dependent
NA activity is often measured using small monovalent reporter substrates like MUNANA or more biologically relevant multivalent substrates such as the glycoprotein fetuin. With MUNANA (Fig. 1a), NA…
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Structural insights into tRNA recognition of the human FTSJ1-THADA complex
Purification of FTSJ1-THADA
The gene encoding FTSJ1 or THADA was PCR-amplified and cloned into a modified pcDNA3.4 vector (Thermo Fisher Scientific, MA, USA) using the primers listed in Supplementary Table 2. FLAG-tag and TEV protease cleavage…
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Assessment of plasma derived microbiome profiles in lung cancer using targeted and whole exome sequencing
Microbial associations with cancer have been a focus of extensive study, with an estimated 20% of cancer cases worldwide linked to infections1. The involvement of specific microbes in cancer development is well-established. For instance,…
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Autonomic modulation of the immune response and implications for CNS malignancies
Langley, J. The Autonomic Nervous System (W. Heffer & Sons Ltd., 1921).
Pick, J. The Autonomic Nervous System: Morphological, Comparative, Clinical, and Surgical Aspects (Lippincott, 1970).
Jänig, W. Integrative Action of the Autonomic Nervous…
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The role of gut microbiota-gonadal axis in ovary activation of Asian honey bee (Apis cerana) queens
Ashonibare, V. J., Akorede, B. A., Ashonibare, P. J., Akhigbe, T. M. & Akhigbe, R. E. Gut microbiota-gonadal axis: the impact of gut microbiota on reproductive functions. Front. Immunol. 15, 1346035 (2024).
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Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
The BacDive database currently (2024.2 release) comprises more than 2.7 million data points covering more than 1000 data fields23. However, data availability varies markedly for the different traits and different taxa. In addition, genome…
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A large-scale curated and filterable dataset for cryo-EM foundation model pre-training
Yip, K. M., Fischer, N., Paknia, E., Chari, A. & Stark, H. Atomic-resolution protein structure determination by cryo-em. Nature 587, 157–161 (2020).
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LMNB2-mediated high PD-L1 transcription triggers the immune escape of hepatocellular carcinoma
Antibody, chemicals, primers, and siRNA/sh RNA sequence information used in this study are listed in Tables S1–S3.
Dual-luciferase reporter assays
Huh7 cells were transiently transfected with Flag-LMNB2 and pCMV-Vector using Lipo6000 Transfection…
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MRG15 alternative splicing regulates CDK1 transcriptional activity in mouse cell senescence and myocardial regeneration
Isolation and culture of mouse embryonic fibroblasts (MEFs)
Mouse embryonic fibroblasts (MEFs) were isolated from E13.5 embryos of C57BL/6 J mice following ethical guidelines. Embryos were collected aseptically and minced into small pieces after…
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Direct visualization of a disorder driven electronic smectic phase in nonsymmorphic square-net semimetal GdSbTe
Spectroscopic imaging of GdSbxTe2-x
GdSbxTe2-x crystallizes in the tetragonal structure (space group P4/nmm) and evolves to the orthorhombic CDW phase (space group Pmmn) when x < 0.8011,12. GdSbxTe2-x becomes…
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