Expression and purification of G protein heterotrimer
G protein heterotrimer was expressed as described14 in Tni cells (Expression Systems; isolated from Trichoplusia ni; no validation or mycoplasma testing) with viruses for Gα, Gβγ and Ric-8a…
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Analysis of hybrid CNN models optimized with metaheuristic algorithms for melanoma detection
Rahman, Z., Hossain, M. S., Islam, M. R., Hasan, M. M. & Hridhee, R. A. An approach for multiclass skin lesion classification based on ensemble learning. Inform. Med. Unlocked 25, 100659 (2021).
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Photonic ‘ski jump’ steers light beam from silicon chip
- NEWS AND VIEWS
Chip-based beam…
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Dynamics of circulating tumour DNA can guide treatment decisions for improved outcomes
- CLINICAL BRIEFINGS
A phase II trial shows that the risk of cancer…
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Intestinal interoceptive dysfunction drives age-associated cognitive decline
Mice
Unless noted otherwise, young female (8 weeks old) and male (4 weeks old) C57BL/6 mice were obtained from The Jackson Laboratory and old mice (18 months old) were acquired from the National Institute on Aging. For co-housing, mice were housed…
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Synergistic optoelectronic and thermoelectric performance in Rb2AsAuBr6 and Rb2AsAuCl6 double perovskites for multifunctional energy conversion
King, G. & Woodward, P. M. Cation ordering in perovskites. J. Mater. Chem. 20(28), 5785–5796. https://doi.org/10.1039/B926757C (2010).
Zhao, X. G. et al. Design of lead-free inorganic halide…
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Bandwidth broadening in cavity-type sound-absorbing metamaterials via additional equivalent stiffness
Hahad, O. et al. Noise and mental health: Evidence, mechanisms, and consequences. J. Expo. Sci. Environ. Epidemiol. https://doi.org/10.1038/s41370-024-00642-5 (2024).
Jacuzzi, G. et al….
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Assembly of helper NLR resistosome clusters upon activation of a coiled-coil NLR
Plant materials and growth conditions
The Arabidopsis thaliana ecotype Col-0 was used as the WT in this study. The mekk1 (salk_052557), mpk4, summ2 (summ2-8) and mekk2 mutants have been reported previously18,61, and the T-DNA insertion mutant…
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Immune evasive DNA donors and recombinases license kilobase-scale writing
Gaj, T., Sirk, S. J. & Barbas, C. F. Expanding the scope of site-specific recombinases for genetic and metabolic engineering. Biotechnol. Bioeng. 111, 1–15 (2014).
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A big-push community intervention reduced rates of child marriage by 80%
Child Marriage (UNICEF, 2022).
State of the Nigerian Girl Report: An Incisive Diagnosis of Child Marriage in Nigeria (Save the Children, 2021).
Asadullah, M. N. & Wahhaj, Z. Early marriage, social networks and the transmission of norms. Economica…
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