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Jones, K. E. et al. Global trends in…
Category: 5. Biology
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A single amino acid mutation alters multiple neutralization epitopes in the respiratory syncytial virus fusion glycoprotein
Cell culture
Henrietta Lack’s (HeLa) cells from American Type Culture Collection (ATCC, CCL2) were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing L-glutamine, glucose, and sodium pyruvate with 10% heat-inactivated fetal bovine…
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Endothelium calls macrophages for destruction
Endothelium calls macrophages for destruction
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Enzymes in secondary pharmacology screening panels: is there room for improvement?
Brennan, R. J. et al. The state of the art in secondary pharmacology and its impact on the safety of new medicines. Nat. Rev. Drug Discov. 23, 525–545 (2024).
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B cells modulate lung antiviral inflammatory responses via the neurotransmitter acetylcholine
Wu, T. T.-H. et al. Interstitial macrophages are a focus of viral takeover and inflammation in COVID-19 initiation in human lung. J. Exp. Med. 221, e20232192 (2024).
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Antibody function predicts viral control in newborn monkeys immunised with an influenza virus HA stem nanoparticle
H1ssF + AddaVax generates a protective response in adult AGMs
While the primary goal in the current study was to determine whether a universal HA stem vaccine is effective in newborns, we first sought to establish the utility of this vaccine…
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SAMD9 senses cytosolic double-stranded nucleic acids in epithelial and mesenchymal cells to induce antiviral immunity
Cell culture, plasmids, and viruses
Sf9 cells (ATCC #CRL-1711) were cultured in Grace’s Insect Medium (Gibco #11605-094) supplemented with 10% heat-inactivated insect cell culture tested fetal bovine serum (Sigma #F4135). High five cells (Gibco…
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Identification of potential SARS-CoV-2 inhibitors among well-tolerated drugs using drug repurposing and in vitro approaches
Real-time cytotoxicity and cell index measurements
The non-toxic maximum concentration for the 27 selected molecules, which will be used in subsequent cell culture studies with Calu-3 and Caco-2 cell lines, was determined using the xCELLigence…
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TEMI: tissue-expansion mass-spectrometry imaging | Nature Methods
Chemicals and materials
Reagents including Optima LC–MS-grade methanol, ethanol, acetonitrile, 2-propanol (IPA), water, and formic acid were purchased from Fisher Scientific; 6-((acryloyl)amino) hexanoic acid succinimidyl ester (AcX), sodium…
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Safety profile of sikamat virus and its oncolytic potential in leukemic cells and cancer stem cells
Cell viability assays
Cancer cells and virus culture maintenance
Vero (ATCC CCL81), THP-1 (ATCC TIB-202), and K562 (ATCC CCL-243) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM), Roswell Park Memorial Institute (RPMI) 1640 Medium,…
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