Category: 5. Biology

Landscape of tissue sample characteristics and single-cell transcriptome data

To determine the cellular heterogeneity and architecture of HGSC, 19 patients with advanced HGSC and 1 healthy control individual were enrolled in this study. In total,…

Reagents

Punicalagin was purchased from Sigma-Aldrich (cat. no. P0023; St. Louis, MO, USA). Dimethyl sulfoxide obtained from Sigma-Aldrich. Antibodies against Nrf2, SQSTM1/p62 (phospho S349), p-ULK1, p-mTOR and p-AMPK were obtained from Abcam…

Materials

FeCl₃•6H₂O, 9,10-diphenylanthracene (DPA), NH₄HCO₃, and methylene blue (MB) were obtained from Macklin. DMEM medium, fetal bovine serum (FBS), and PBS were obtained from Coning. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zolium…

Methods of reporter analysis, cell culture, real-time quantitative RT-PCR, in vivo EV-uptake study and histological analyses are described in Supplementary Methods.

EV isolation

EVs were collected from the medium using ExoQuick-TC Exosome…

Reduction of microbial swimming speed under microconfinement

We developed microfluidic devices to mimic the intricate geometry of flooded pores in soil. However, it must be remembered that these devices are intended as approximations to simulate…