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Wilkinson, A. C….
Category: 5. Biology
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IGF2BP3 recruits NUDT21 to regulate SPTBN1 alternative polyadenylation and drive ovarian cancer progression
Clinical tissue sample collection
Patients diagnosed with OC were enrolled in this study, and all of the cancer and control samples were collected from OC patients. The clinical samples consist of 12 cases of high-grade serous ovarian cancer…
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Immunotherapy for autoimmune encephalitis | Cell Death Discovery
Papi C, Milano C, Spatola M. Mechanisms of autoimmune encephalitis. Curr Opin Neurol. 2024;37:305–15.
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Sakharova T, Aringazina R, Lilyanov N, Monov D. Features of the…
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Application of a sunlight-switched sugar signal increases wheat yield in the field
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This is a summary of: Griffiths, C. A. et al. Membrane-permeable trehalose 6-phosphate precursor spray…
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Predicting rare drug-drug interaction events with dual-granular structure-adaptive and pair variational representation
Dataset description
For the problem of drug-drug interaction events prediction, we mainly conduct experiments on the two public datasets: DrugBank54 and TWOSIDES55. The former is mainly used as the benchmark data set for comparison with SOTA, and…
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Membrane-permeable trehalose 6-phosphate precursor spray increases wheat yields in field trials
Optimized DMNB-T6P synthesis
General chemical materials and methods
All reagents were purchased from commercial sources and were used without further purification unless noted. Molecular sieve (4 Å, powder) used in reactions was activated at…
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Amphiphilic mPEG-PLGA copolymer nanoparticles co-delivering colistin and niclosamide to treat colistin-resistant Gram-negative bacteria infections
Materials
The mcr-1 positive colistin-resistant bacteria including Salmonella Typhimurium (Salmonella SH05, Salmonella 2a), E. coli (E. coli Y150, E. coli E31), and K. pneumoniae (K. pneumoniae 5G, K. pneumoniae 3Q) were clinically…
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Enabling next-generation anaerobic cultivation through biotechnology to advance functional microbiome research
Hungate, R. E., Smith, W. & Clarke, R. T. Suitability of butyl rubber stoppers for closing anaerobic roll culture tubes. J. Bacteriol. 91, 908–909 (1966).
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Mechanistic studies of mycobacterial glycolipid biosynthesis by the mannosyltransferase PimE
Overexpression and purification of MaPimE
WT MaPimE was cloned into pNYCOMPSC23 plasmid65. The procedure for the design and synthesis of pNYCOMPSC23 plasmid was described in detail in a previously published protocol65. The plasmids were…
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