CTC and PBMC isolation and cell line establishment
Patients with plurimetastatic gastrointestinal cancer were enrolled at the IRCCS Istituto Romagnolo per lo Studio dei Tumori (IRST) “Dino Amadori” (Italy) between 2015 and 2019 (N = 24)….
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Cell differentiation, aging, and death in spatially organized yeast communities: mechanisms and consequences
Fuchs Y, Steller H. Programmed cell death in animal development and disease. Cell. 2011;147:742–58.
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Galluzzi L, Vitale I, Aaronson SA, Abrams JM, Adam…
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Shifts in sex-specific immune gene expression in a beetle with parental care
Abbas, A. K., Lichtman, A. H. & Pillai, S. Cellular and Molecular Immunology (Elsevier, Amsterdam, 2021).
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Bird, L. Getting enough energy for immunity. Nat. Rev. Immunol. 19,…
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Membrane-targeted immunogenic compositions using exosome mimetic approach for vaccine development against SARS-CoV-2 and other pathogens
SARS-CoV-2 S Δ21 protein overexpression localizes Spike protein to the cell membrane
We examined the expression of the SARS-CoV-2 S protein in cells expressing either the SARS-CoV-2-S Δ21 variant or the wild type (wt) using the pRetroX vector…
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Enhancing nucleotide sequence representations in genomic analysis with contrastive optimization
Overview of Scorpio
As an initial dataset to evaluate the Scorpio framework, we curated a set of 800,318 sequences. First, we used 1929 bacterial and archaeal genomes curated using the Woltka pipeline27, each representing a single genus and with a…
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β-Carotene alleviates substrate inhibition caused by asymmetric cooperativity
Carotenoids and xanthophylls are effectors of Nicotiana benthamiana glucosyltransferase NbUGT72AY1
NbUGT72AY1 is a promiscuous transferase that glycosylates bioactive hydroxycoumarins such as scopoletin and umbelliferone, as well as monolignols…
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The 626M24 dataset of validated transitions and empirical rovibrational energy levels of 16O12C16O
Pearson, P. N. & Palmer, M. R. Atmospheric carbon dioxide concentrations over the past 60 million years. Nature 406, 695–699, https://doi.org/10.1038/35021000 (2000).
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Chronic disturbance alters seed dispersal traits and frugivores resources in a dry tropical forest
Vitousek, P. Beyond global warming: ecology and global change. Ecology 75, 1861–1876 (1994).
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Ribeiro, E. M. S. et al. Functional diversity and composition of Caatinga Woody flora…
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Vorinostat attenuates UVB-induced skin senescence by modulating NF-κB and mTOR signaling pathways
Chemicals and reagents
Vorinostat (SAHA) (S1047) was purchased from Selleck Chemicals (Shanghai, China). Hematoxylin–Eosin staining kit (Cat# G1120) was obtained from Solarbio (Beijing, China). Senescence-associated β-galactosidase (SA-β-Gal)…
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The impact of initiation timing of continuous renal replacement therapy on outcomes in critically ill patients with acute kidney injury a retrospective study from the MIMIC-IV database
Study design and setting
We performed a retrospective observational cohort study at a single institution, utilizing data from the Medical Information Mart for Intensive Care IV version 2.2 (MIMIC-IV v2.2)12, a comprehensive publicly accessible…
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