Antibodies and reagents
The anti-GAPDH (AC054) antibody and anti-β-Actin (AC028) antibody were obtained from ABclonal Technology. The anti-Caspase-1 P20 (AG-20B-0042) antibody and anti-NLRP3 (AG-20B-0006) antibody were purchased from Adipogen…
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Neuronal CDK5RAP3 deficiency leads to encephalo-dysplasia via upregulation of N-glycosylases and glycogen deposition
Kukuruzinska MA, Lennon K. Protein N-glycosylation: molecular genetics and functional significance. Crit. Rev. Oral. Biol. Med. 1998;9:415–48.
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Wilson CM, Roebuck Q, High S….
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Uncovering critical transitions and molecule mechanisms in disease progressions using Gaussian graphical optimal transport
The details of the theoretical background and related work can be found in Supplementary Section A.
Gaussian graphical model
Gaussian graphical models22 are probabilistic graphical models, which can represent the conditional dependencies between…
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Spatially informed graph transformers for spatially resolved transcriptomics
Overview of SpaGT
We developed SpaGT, a spatially informed graph transformer framework designed specifically for spatially resolved transcriptomics (SRT) analysis. SpaGT enables the learning of spatially aware graph representation for denoising…
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Pharmacological targeting of the mitochondrial phosphatase PTPMT1 sensitizes hepatocellular carcinoma to ferroptosis
All animal experiments were conducted in strict compliance with ethical standards and were approved by the Biomedical Ethics Committee of the Health Science Center of Xi’an Jiaotong University (Approval number: 2022–1371) on June 9th 2022….
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Targeting alveolar epithelial cells with lipid micelle-encapsulated necroptosis inhibitors to alleviate acute lung injury
Mice
Male C57BL/6 mice, aged 6-8 weeks, weighing between 16-24 grams, were purchased from SHANGHAI SLAC LABORATORY ANIMAL CO.LTD., and were acclimated for one week in the animal laboratory of the Clinical Research Center of the Second Affiliated…
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Initiation of enteral feeding as per current protocol (at 24 h) versus as per clinical status (from 6 h onwards) in neonates born with antenatal reversed end diastolic flow (REDF) in the umbilical artery Doppler: a pilot randomized controlled trial
Dorling J, Kempley S, Leaf A. Feeding growth restricted preterm infants with abnormal antenatal Doppler results. Arch Dis Child Fetal Neonatal Ed. 2005;90:F359–363.
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Cross the borders: the dual cultural devaluation of the Belt and Road co-production films
Basic features
During the first decade of the BRI, China collaborated on 92 cinematic co-productions with countries along the route, generating approximately $830 million in domestic box office revenue and $1.1 billion globally. Figure 1…
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ESM-Ezy: a deep learning strategy for the mining of novel multicopper oxidases with superior properties
Materials
All chemicals used in this study were of analytical grade or higher. Plasmids for the expression of various multicopper oxidases (MCOs) were synthesized by SynbioB (Tianjin, China). LB broth powder, Isopropyl β-d-1-thiogalactopyranoside…
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Warfarin use and vestibular dysfunction insights from NHANES data, network pharmacology, Mendelian randomization, and molecular docking
This study explored the relationship and potential mechanisms between warfarin ues and vestibular dysfunction through a multi-stage approach. First, we investigated the association between warfarin use and vestibular dysfunction using the NHANES…
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