Molecular structure and enzymatic mechanism of the human collagen hydroxylysine galactosyltransferase GLT25D1/COLGALT1

GLT25D1/COLGALT1 is a Mn²⁺ dependent galactosyltransferase

We established methods to recombinantly produce and purify human full-length GLT25D1/COLGALT1 in E. coli and obtained enzyme preparations suitable for evaluation of galactosyltransferase…