Engineering intercellular communication using M13 phagemid and CRISPR-based gene regulation for multicellular computing in Escherichia coli

Establishing a M13 phagemid system for intercellular communication

For gene regulation in our engineered microbial consortium of E. coli strains we employ CRISPRi^21,34. To establish communication between consortium members, we combined CRISPRi…