Overall structure of Rat1-Rai1-Rtt103 ternary complex
To produce the Rat1-Rai1-Rtt103 complex, we first tried to express the three proteins separately in E. coli and assemble them in vitro. Although Rat1 and Rtt103 could be expressed and purified,…
To produce the Rat1-Rai1-Rtt103 complex, we first tried to express the three proteins separately in E. coli and assemble them in vitro. Although Rat1 and Rtt103 could be expressed and purified,…