Human iPSC culture
iPSCs were cultured on Matrigel (Corning; Cat. No. 356231) coated vessels in StemFlex Media (Gibco; Cat. No. A3349401) until 80% confluent. Upon confluency, cells were washed with DPBS (Gibco; Cat. No. 14190-144), lifted using…
CRISPRi-based screen of autism spectrum disorder risk genes in microglia uncovers roles of ADNP in microglia endocytosis and synaptic pruning
