mESC culture maintenance and spontaneous differentiation
E14 mESCs (gift from the Panning lab, UCSF) were maintained on gelatin coated dishes in 2i+LIF media, composed of a 1:1 mixture of DMEM/F12 (Thermo Fisher, 11320-033) and Neurobasal (Thermo…
E14 mESCs (gift from the Panning lab, UCSF) were maintained on gelatin coated dishes in 2i+LIF media, composed of a 1:1 mixture of DMEM/F12 (Thermo Fisher, 11320-033) and Neurobasal (Thermo…