Structural analyses define the molecular basis of clusterin chaperone function

Plasmids

The pB-T-PAF-Clu plasmid used for WT Clu expression and purification was constructed by amplifying the coding region of pB-T-PAF-CluStrep²² by PCR. The PCR product was then digested with NheI and NotI and subcloned into the pB-T-PAF…