Pathogenic morphological signatures of perturbations in mitochondrial-related genes revealed by pooled imaging assay

Culture and transfection

Human osteosarcoma U2OS cell line (U2OS, ATCC HTB-96) with doxycycline-inducible Cas9¹³ was cultured in McCoy’s 5 A Modified Medium (16600082, Gibco, Gaithersburg, MD, USA) supplemented with 10% tetracycline-free fetal…