Cell culture
Cerebral microvascular endothelial cells (HBEC 5i) were purchased from ATCC (CRL-3245, USA) and cultured up to the 9th passage in DMEM/F12 (Gibco, UK) medium supplemented with 10% fetal bovine serum (FBS, Gibco, UK),…
Cerebral microvascular endothelial cells (HBEC 5i) were purchased from ATCC (CRL-3245, USA) and cultured up to the 9th passage in DMEM/F12 (Gibco, UK) medium supplemented with 10% fetal bovine serum (FBS, Gibco, UK),…