Marker-independent vibrational spectroscopy imaging recognizes the hypoxia effect in the human brain endothelium

Cell culture

Cerebral microvascular endothelial cells (HBEC 5i) were purchased from ATCC (CRL-3245, USA) and cultured up to the 9th passage in DMEM/F12 (Gibco, UK) medium supplemented with 10% fetal bovine serum (FBS, Gibco, UK),…

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