Generic comparison of lumen nucleation and fusion in epithelial organoids with and without hydrostatic pressure

Cell sources and expansion

We used 3 cellular systems, MDCK II cell lines, mouse embryonic cells (mES cells)⁴³, and pancreatic spheres⁴⁴. Other mutant cell lines were used for MDCK: MDCK II E-cadherin-GFP/Podocalyxin- mScarlett/Halo-CAAX⁴⁵, MDCK…