Microfluidics combined with electron microscopy for rapid and high-throughput mapping of antibody–viral glycoprotein complexes

Protein purification and expression

For protein expression, FreeStyle 293F cells were transfected at a density of 1 × 10⁶ cells ml⁻¹ as previously described⁴⁰. In brief, PEI-MAX (1 mg ml⁻¹) was mixed with 250 µg of HIV…