Plasmids and strains
In this study, all plasmids were constructed by Gibson assembly. The products (homologous arms, target genes) obtained via polymerase chain reaction (PCR) were digested, recovered, and then ligated to the pUC-HUH vector…
In this study, all plasmids were constructed by Gibson assembly. The products (homologous arms, target genes) obtained via polymerase chain reaction (PCR) were digested, recovered, and then ligated to the pUC-HUH vector…